PXD027757-1

PXD027757 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Mitochondrial interactome quantitation reveals structural changes in metabolic machinery in failing murine heart
Description	Advancements of cross-linking mass spectrometry (XL-MS) for structural analysis of proteins bridges the gap between purified systems and native tissue environments. Here, we utilize isobaric quantitative protein interaction reporter technology (iqPIR) to further extend XL-MS to the first system-wide comparative study of mitochondrial proteins from healthy and diseased murine hearts. The failing heart interactome includes 544 statistically significant cross-linked peptide pairs altered in disease condition. Structural insight into ketone oxidation metabolons, OXPHOS machinery, and nucleotide transporter hybrid-conformations, support mitochondrial remodeling in failing heart while bringing forth new hypotheses for pathological mechanisms. Application of quantitative cross-linking technology in live tissue provides molecular-level insight to complex biological systems difficult to model in cell culture, thus providing a valuable resource for study of human diseases.
HostingRepository	PRIDE
AnnounceDate	2022-08-02
AnnouncementXML	Submission_2022-08-02_03:36:35.753.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Xiaoting Tang
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-05 10:01:28	ID requested
⏵ 1	2022-08-02 03:36:36	announced

Publication List

Dataset with its publication pending

Dataset with its publication pending

Keyword List

submitter keyword: quantitative cross-linking
heart failure
cardiac hypertrophy
mitochondria
protein interactions
interactome
mass spectrometry
systems structural biologyquantitative cross-linking
heart failure
cardiac hypertrophy
mitochondria
protein interactions
interactome
mass spectrometry
systems structural biology

submitter keyword: quantitative cross-linking

heart failure

cardiac hypertrophy

mitochondria

protein interactions

interactome

mass spectrometry

systems structural biologyquantitative cross-linking

heart failure

cardiac hypertrophy

mitochondria

protein interactions

interactome

mass spectrometry

systems structural biology

Contact List

James Edward Bruce
contact affiliation	Department of Genome Sciences, University of Washington
contact email	jimbruce@uw.edu
lab head
Xiaoting Tang
contact affiliation	University of Washington
contact email	xiaoting@uw.edu
dataset submitter

Full Dataset Link List

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD027757
PRIDE project URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD027757

PRIDE project URI

Repository Record List

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