PXD027723 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mass spectrometric and bio-computational analysis of multiply charged RNase S gas phase complexes obtained by electrospray ionization from varying in-solution equilibrium conditions |
Description | In this project we investigate the influence of a solvent's composition on the stability of desorbed and multiply charged RNase S ions by analyzing gas phase dissociation reactions triggered by collision induced dissociation (CID) of multiply charged complex ions. RNase S was dissolved in ESI-compatible buffers with either an increasing content of organic co-solvent or with different pH. Direct transition of all ions from the in-solution components is followed by CID of the non-covalent RNase S complex and by quantitative analysis of the dissociation products. From normalized ion abundances are determined the apparent kinetic and apparent thermodynamic gas phase complex properties. The stability of RNase S in the gas phase is independent from the in-solution equilibrium but sensitive to differences in charge states. |
HostingRepository | PRIDE |
AnnounceDate | 2021-11-23 |
AnnouncementXML | Submission_2021-11-23_03:49:43.809.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Michael Kreutzer |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | No PTMs are included in the dataset |
Instrument | Waters instrument model |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-05 08:58:08 | ID requested | |
⏵ 1 | 2021-11-23 03:49:44 | announced | |
Publication List
Koy C, Opuni KFM, Danquah BD, Neamtu A, Glocker MO, Mass Spectrometric and Bio-Computational Binding Strength Analysis of Multiply Charged RNAse S Gas-Phase Complexes Obtained by Electrospray Ionization from Varying In-Solution Equilibrium Conditions. Int J Mol Sci, 22(19):(2021) [pubmed] |
Keyword List
submitter keyword: Off-line ESI-MS, ITEM-TWO, RNase S, complex stability, varying content of MeOH co-solvent, different pH |
Contact List
Prof. Dr. Michael O. Glocker |
contact affiliation | Proteome Center Rostock, University of Rostock, Germany |
contact email | michael.glocker@med.uni-rostock.de |
lab head | |
Michael Kreutzer |
contact affiliation | University Medical Center Rostock |
contact email | michael.kreutzer@med.uni-rostock.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027723
- Label: PRIDE project
- Name: Mass spectrometric and bio-computational analysis of multiply charged RNase S gas phase complexes obtained by electrospray ionization from varying in-solution equilibrium conditions