PXD027722-1

PXD027722 is an original dataset announced via ProteomeXchange.

Title	Dynamic human liver proteome atlas reveals functional insights into disease pathways
Description	Deeper understanding of liver pathophysiology would benefit from a comprehensive quantitative proteome resource at cell-type resolution. Here, we quantify more than 10,000 proteins and 150,000 sequence-unique peptides across total liver, the major liver cell types, time-course of primary cell cultures and liver disease states. Bioinformatic analysis reveals that half of hepatocyte protein mass is comprised of enzymes and 23% of mitochondrial proteins, twice the proportion of other liver cell types. Using primary cell cultures, we capture dynamic proteome remodeling from tissue states to cell line states, providing useful information for biological or pharmaceutical research. Our extensive data serves as spectral library to characterize a human cohort of non-alcoholic steatohepatitis and cirrhosis. Dramatic proteome changes in liver biopsies include signatures of stellate cell activation resembling liver cirrhosis, providing functional insights.
HostingRepository	PRIDE
AnnounceDate	2022-05-08
AnnouncementXML	Submission_2022-05-08_09:25:19.365.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Lili Niu
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480; Q Exactive HF-X

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-05 08:58:08	ID requested
⏵ 1	2022-05-08 09:25:19	announced

Dataset with its publication pending

submitter keyword: Human, liver, atlas, primary cell culture, liver disease, NASH, cirrhosis

Matthias Mann
contact affiliation	Novo Nordisk Foundation Center for Protein Research, Faculty of Health Sciences, University of Copenhagen, DK-2200 Copenhagen, Denmark
contact email	mmann@biochem.mpg.de
lab head
Lili Niu
contact affiliation	University of Copenhagen
contact email	lili.niu@cpr.ku.dk
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD027722

PRIDE project URI

• PRIDE
  1. PXD027722
     1. Label: PRIDE project
     2. Name: Dynamic human liver proteome atlas reveals functional insights into disease pathways