PXD027721 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | We applied an iodoacetyl tandem mass tags (iodoTMT)-based proteomic approach to analyze the redox proteome of Brassica napus seedlings under control and salt-stressed conditions. We identified 1,821 sulfenylated sites in 912 proteins from all samples. |
Description | By means of large-scale redox proteomics, we studied reversible cysteine modification during the response to short-term salt stress in Brassica napus. We applied an iodoacetyl tandem mass tags (iodoTMT)-based proteomic approach to analyze the redox proteome of Brassica napus seedlings under control and salt-stressed conditions. We identified 1,821 sulfenylated sites in 912 proteins from all samples. A great number of sulfenylated proteins were predicted to localize to chloroplasts and cytoplasm and GO enrichment analysis of differentially sulfenylated proteins revealed that metabolic processes such as photosynthesis and glycolysis are enriched and enzymes are overrepresented. Redox-sensitive sites in two enzymes were validated in vitro on recombinant proteins and they might affect the enzyme activity. This targeted approach contributes to the identification of the sulfenylated sites and proteins in Brassica napus subjected to salt stress and our study will improve our understanding of the molecular mechanisms underlying the redox regulation in response to salt stress. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-18 |
AnnouncementXML | Submission_2021-08-18_05:58:38.840.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Liangqian Yu |
SpeciesList | scientific name: Brassica napus (Rape); NCBI TaxID: 3708; |
ModificationList | monohydroxylated residue |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-08-05 05:52:52 | ID requested | |
⏵ 1 | 2021-08-18 05:58:39 | announced | |
2 | 2021-08-18 06:06:27 | announced | 2021-08-18: Updated publication reference for PubMed record(s): 34347306. |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Brassica napus, leaf, LC-MS/MS |
Contact List
Liang Guo |
contact affiliation | National Key Laboratory of Crop Genetic Improvement, Huazhong Agricultural University |
contact email | guoliang@mail.hzau.edu.cn |
lab head | |
Liangqian Yu |
contact affiliation | 1534447256@qq.com |
contact email | 1534447256@qq.com |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD027721 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027721
- Label: PRIDE project
- Name: We applied an iodoacetyl tandem mass tags (iodoTMT)-based proteomic approach to analyze the redox proteome of Brassica napus seedlings under control and salt-stressed conditions. We identified 1,821 sulfenylated sites in 912 proteins from all samples.