PXD027708-1

PXD027708 is an original dataset announced via ProteomeXchange.

Title	Analysis of RNA polymerase II interacting proteins in wild-type and spt6-1004 yeast cells.
Description	The histone chaperone Spt6 is involved in promoting elongation of RNA polymerase II (RNAPII), maintaining chromatin structure, regulating co-transcriptional histone modifications, and controlling mRNA processing. These diverse functions of Spt6 are partly mediated through its interactions with RNAPII and other factors in the transcription elongation complex. In this study, we used mass spectrometry to characterize the differences in RNAPII interacting factors between wild-type cells and those depleted for Spt6, leading to the identification of proteins that depend on Spt6 for their interaction with RNAPII. In all, eight samples were processed - four genotypes (1. SPT6, RPB3-untagged; 2. SPT6, RPB3-tagged; 3. spt6-1004, RPB3-untagged; 4. spt6-1004; RPB3-tagged) in biological duplicates.
HostingRepository	PRIDE
AnnounceDate	2021-12-05
AnnouncementXML	Submission_2021-12-05_14:55:53.789.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027708
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Rajaraman Gopalakrishnan
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-05 04:38:50	ID requested
⏵ 1	2021-12-05 14:55:54	announced

Gopalakrishnan R, Winston F, The histone chaperone Spt6 is required for normal recruitment of the capping enzyme Abd1 to transcribed regions. J Biol Chem, 297(4):101205(2021) [pubmed]

submitter keyword: S. cerevisiae, TMT, Spt6, RNAPII

Fred Winston
contact affiliation	Department of Genetics, Harvard Medical School, Boston MA 02115 USA
contact email	winston@genetics.med.harvard.edu
lab head
Rajaraman Gopalakrishnan
contact affiliation	Harvard Medical School
contact email	rajaraman.g@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027708
     1. Label: PRIDE project
     2. Name: Analysis of RNA polymerase II interacting proteins in wild-type and spt6-1004 yeast cells.