PXD027705-1

PXD027705 is an original dataset announced via ProteomeXchange.

Title	Regulated biogenesis and targeting of monotopic squalene monooxygenase requires the GET pathway
Description	This study investigated get3 mutant yeast cell. Proteins of the GET pathway are involved in targeting of C-terminally anchored transmembrane proteins and protection against lipotoxicity. Get3 cells revealed an altered ergosterol production and susceptibility towards a sterol synthesis inhibiting drug terbinafine. Furthermore, we identified a member of a non-canonical GET pathway client, a monotopic membrane protein squalene monooxygenase Erg1 that may be responsible for the susceptibility of Get3 mutant to lipotoxic agents.
HostingRepository	PRIDE
AnnounceDate	2022-06-09
AnnouncementXML	Submission_2022-06-09_07:15:38.776.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Ivan Silbern
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-08-05 04:38:24	ID requested
⏵ 1	2022-06-09 07:15:39	announced

Farkas Á, Urlaub H, Bohnsack KE, Schwappach B, Regulated targeting of the monotopic hairpin membrane protein Erg1 requires the GET pathway. J Cell Biol, 221(6):(2022) [pubmed]

submitter keyword: squalene monooxygenase, GET pathway, Get3, Erg1, lipotoxicity, terbinafine, LFQ, in-gel digestion

Prof. Dr. Henning Urlaub
contact affiliation	Bioanalytical Mass Spectrometry Group, Max-Planck Institute for Biophysical Chemistry, Am Fassberg 11, 37077 Goettingen, Germany
contact email	henning.urlaub@mpibpc.mpg.de
lab head
Ivan Silbern
contact affiliation	MPI Biophysical Chemistry
contact email	ivansilbern@gmail.com
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027705
     1. Label: PRIDE project
     2. Name: Regulated biogenesis and targeting of monotopic squalene monooxygenase requires the GET pathway