PXD027640-1

PXD027640 is an original dataset announced via ProteomeXchange.

Title	The Mediator complex in the malaria parasite Plasmodium falciparum contains evolutionarily novel subunits
Description	The eukaryote Mediator is a large and conserved multi-subunit protein complex that directly contacts RNA polymerase II and impinges on multiple aspects of gene expression. The genome of the human malaria parasite P. falciparum has been predicted to encode several Mediator subunits. We provide physical evidence for the presence of a Mediator complex in P. falciparum by using co-immunoprecipitation and mass spectrometry to identify interaction partners of the highly conserved Mediator subunit, PfMed31. We identify 11 of 14 predicted Mediator subunits as well as the products of two uncharacterized genes, PF3D7_0526800 and PF3D7_1363600, which strongly associate with PfMed31. As expected, several additional interaction partners have known roles in the transcriptional control of gene expression and mRNA processing. Intriguingly, multiple interaction partners are implicated in endoplasmic reticulum function and the ER stress (ERS) response suggesting crosstalk between the ERS response and transcriptional machineries. Our results establish for the first time the physical presence of the Mediator complex within P. falciparum, and strongly suggest that it plays both conserved and unique roles in the control of gene expression.
HostingRepository	PRIDE
AnnounceDate	2022-05-10
AnnouncementXML	Submission_2022-05-10_06:21:23.818.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	SoFong Cam Ngan
SpeciesList	scientific name: Plasmodium falciparum 3D7; NCBI TaxID: 36329;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-29 02:01:06	ID requested
⏵ 1	2022-05-10 06:21:24	announced
2	2022-05-16 02:55:36	announced	2022-05-16: Updated publication reference for PubMed record(s): 35557691.

Dataset with its publication pending

submitter keyword: Plasmodium falciparum

interactome

Mark Featerstone
contact affiliation	Nanyang Technological University
contact email	msfeatherstone@gmail.com
lab head
SoFong Cam Ngan
contact affiliation	Nanyang Technological University
contact email	sfcngan@ntu.edu.sg
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/05/PXD027640

PRIDE project URI

• PRIDE
  1. PXD027640
     1. Label: PRIDE project
     2. Name: The Mediator complex in the malaria parasite Plasmodium falciparum contains evolutionarily novel subunits