PXD027638-1

PXD027638 is an original dataset announced via ProteomeXchange.

Title	The human innate immune protein calprotectin elicits a multi-metal starvation response in Pseudomonas aeruginosa
Description	To combat infections, the mammalian host limits availability of essential transition metals such as iron (Fe), zinc (Zn), and manganese (Mn) in a strategy termed “nutritional immunity”. The innate immune protein calprotectin (CP) contributes to nutritional immunity by sequestering these metals to exert antimicrobial activity against a broad range of microbial pathogens. One such pathogen is Pseudomonas aeruginosa, which causes opportunistic infections in vulnerable populations including individuals with cystic fibrosis. CP was previously shown to withhold Fe(II) and Zn(II) from P. aeruginosa and induce Fe- and Zn-starvation responses in this pathogen. In this work, we performed quantitative, label-free proteomics to further elucidate how CP impacts metal homeostasis pathways in P. aeruginosa. We report that CP induces an incomplete Fe-starvation response, as many Fe-containing proteins that are repressed by Fe limitation are not affected by CP treatment. The Zn-starvation response elicited by CP seems to be more complete than the Fe-starvation response and includes increases in Zn transporters and Zn-independent proteins. CP also induces the expression of membrane-modifying enzymes, and metal-depletion studies indicate this response results from the sequestration of multiple metals. Moreover, the increased expression of membrane-modifying enzymes upon CP treatment correlates with increased tolorance to polymyxin B. Thus, response of P. aeruginosa to CP treatment includes both single and multi-metal starvation responses and includes many factors related to virulence potential, broadening our understanding of this pathogen’s interaction with the host.
HostingRepository	PRIDE
AnnounceDate	2021-07-29
AnnouncementXML	Submission_2021-07-29_10:58:13.525.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Weiliang Huang
SpeciesList	scientific name: Pseudomonas aeruginosa PA14; NCBI TaxID: 652611;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-29 02:00:46	ID requested
⏵ 1	2021-07-29 10:58:13	announced
2	2024-10-22 05:24:22	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: innate immune, calprotectin, multi-metal starvation, Pseudomonas aeruginosa

Maureen Kane
contact affiliation	Mass spectrometry research center, School of pharmacy University of Maryland, Baltimore
contact email	mkane@rx.umaryland.edu
lab head
Weiliang Huang
contact affiliation	University of Maryland, Baltimore
contact email	whuang@rx.umaryland.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/07/PXD027638

PRIDE project URI

• PRIDE
  1. PXD027638
     1. Label: PRIDE project
     2. Name: The human innate immune protein calprotectin elicits a multi-metal starvation response in Pseudomonas aeruginosa