PXD027617 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Targeted Protein Acetylation in Cells Using Heterobifunctional Molecules |
Description | Protein acetylation is a central event in orchestrating diverse cellular processes. However, current strategies to investigate protein acetylation in cells are often non-specific or lack temporal and magnitude control. Here, we developed an acetylation tagging system, AceTAG, to induce acetylation of targeted proteins. The AceTAG system utilizes bifunctional molecules to direct the lysine acetyltransferase p300/CBP to proteins fused with the small protein tag FKBP12F36V, resulting in their induced acetylation. Using AceTAG, we induced targeted acetylation of a diverse array of proteins in cells, specifically histone H3.3, the NF-B subunit p65/RelA, and the tumor suppressor p53. We demonstrate that targeted acetylation with the AceTAG system is rapid, selective, reversible, and can be controlled in a dose-dependent fashion. AceTAG represents a useful strategy to modulate protein acetylation and will enable the exploration of targeted acetylation in basic biological and therapeutic contexts. |
HostingRepository | PRIDE |
AnnounceDate | 2024-01-26 |
AnnouncementXML | Submission_2024-01-26_07:05:52.707.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Li Yun Chen |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; iodoacetamide derivatized residue |
Instrument | Orbitrap Fusion Lumos |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-28 02:30:27 | ID requested | |
⏵ 1 | 2024-01-26 07:05:53 | announced | |
Publication List
Wang WW, Chen LY, Wozniak JM, Jadhav AM, Anderson H, Malone TE, Parker CG, Targeted Protein Acetylation in Cells Using Heterobifunctional Molecules. J Am Chem Soc, 143(40):16700-16708(2021) [pubmed] |
10.1021/jacs.1c07850; |
Keyword List
submitter keyword: targeted acetylation, acetylproteomics, p300/CBP, heterobifunctional molecules |
Contact List
Christopher G. Parker |
contact affiliation | Assistant Professor, Department of Chemistry, The ScrippsResearch Institute |
contact email | cparker@scripps.edu |
lab head | |
Li Yun Chen |
contact affiliation | The Scripps Research Institute |
contact email | lichen@scripps.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2024/01/PXD027617 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027617
- Label: PRIDE project
- Name: Targeted Protein Acetylation in Cells Using Heterobifunctional Molecules