PXD027609-1

PXD027609 is an original dataset announced via ProteomeXchange.

Title	Low temperature exposure has immediate and lasting effects on the stress tolerance, chemotaxis and proteome of entomopathogenic nematodes
Description	Entomopathogenic nematodes (EPN) such as Steinernema spp. and Heterorhabditis spp. are obligate parasites of insects and effective biocontrol agents. Infective juveniles (IJs) of Steinernema carpocapsae and Heterorhabditis megidis are distantly related and exhibit different foraging behaviours but utilise similar volatiles to find hosts. These IJs live in the soil year-round and are exposed to a range of temperatures and associated stressors. Here we attempt to understand how exposure to low temperatures, and the duration of this exposure, affects the behaviour and stress resistance of these IJs. S. carpocapsae and H. megidis have similar responses to many odorants upon emergence from their natal host. The chemotaxis of H. megidis at 20°C is generally enhanced after exposure to lower temperatures, whereas the chemotaxis of S. carpocapsae at 20°C tends to be inverted by exposure low temperatures. Exposure to temperatures below culture temperature affects the chemotaxis of H. megidis gradually, whereas S. carpocapsae behave in a binary manner. Brief exposure to 9°C causes the behaviour of S. carpocapsae IJs to be altered and increased their resistance to freezing and desiccation for weeks afterwards. Label free quantitative proteomics confirms that exposure to 9°C for 1 week elicits dramatic increases in chaperone proteins in S. carpocapsae IJs, which are retained at high levels for 2 weeks after their transfer to 20°C. The proteome of S. carpocapsae IJs exposed to 9°C for 1 week and subsequently transferred to 20°C for 2 weeks conforms closely to that of IJs kept at 9°C for 3 weeks. Thus, S. carpocapsae IJs display a high degree of plasticity, and their behaviour and stress resistance may be manipulated by brief controlled exposure to low temperatures.
HostingRepository	PRIDE
AnnounceDate	2022-11-09
AnnouncementXML	Submission_2022-11-09_03:25:30.571.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	JamesCarolan
SpeciesList	scientific name: Escherichia coli; NCBI TaxID: 562; scientific name: Steinernema carpocapsae; NCBI TaxID: 34508;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-27 22:36:04	ID requested
⏵ 1	2022-11-09 03:25:30	announced

Dataset with its publication pending

submitter keyword: chemotaxis, proteomics, conditioning,temperature, biological pest control

JamesCarolan
contact affiliation	Maynooth University, Ireland
contact email	james.carolan@mu.ie
lab head
JamesCarolan
contact affiliation	Biology
contact email	james.carolan@nuim.ie
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027609
     1. Label: PRIDE project
     2. Name: Low temperature exposure has immediate and lasting effects on the stress tolerance, chemotaxis and proteome of entomopathogenic nematodes