PXD027543-1

PXD027543 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Oil-Roasted Cashew Using a Customized Database
Description	Cashew is one of the most prevalent causes of tree nut allergies. However, the cashew proteome is far from complete, which limits the quality of peptide identification in mass spectrometric analyses. In this study, bioinformatics tools were utilized to construct a customized cashew protein database and improve sequence quality for proteins of interest, based on a publicly available cashew genome database. As a result, two additional isoforms for cashew 2S albumins and five other isoforms for cashew 11S proteins were identified, along with several other potential allergens. Using the optimized protein database, the protein profiles of cashew nuts subjected to different oil-roasting conditions (138 °C and 166 °C for 2-10 minutes) were analyzed using discovery LC-MS/MS analysis. The results showed that cashew 2S protein is most heat-stable, followed by 11S and 7S proteins, though protein isoforms might be affected differently. Preliminary target peptide selection indicated that out of the 29 potential targets, 18 peptides were derived from the newly developed database. In the evaluation of thermal processing effects on cashew proteins, several Maillard reaction adducts were also identified. The cashew protein database developed in this study allows for comprehensive analyses of cashew proteome and development of high-quality allergen detection method.
HostingRepository	PRIDE
AnnounceDate	2022-06-07
AnnouncementXML	Submission_2022-06-07_02:08:13.136.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027543
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Shimin Chen
SpeciesList	scientific name: Anacardium occidentale; NCBI TaxID: 171929;
ModificationList	carbamoylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF; Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-25 15:33:55	ID requested
⏵ 1	2022-06-07 02:08:13	announced

Dataset with its publication pending

submitter keyword: Cashew, Proteomics, Proteogenomics, Oil roasting, Allergen

Melanie Downs
contact affiliation	Department of Food Science and Technology, University of Nebraska-Lincoln
contact email	mdowns2@unl.edu
lab head
Shimin Chen
contact affiliation	University of Nebraska-Lincoln
contact email	shimin@huskers.unl.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027543
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Oil-Roasted Cashew Using a Customized Database