PXD027531-1

PXD027531 is an original dataset announced via ProteomeXchange.

Title	MHCI peptidome of HepG2 with ArgRS or SRRM2 knock down
Description	Cells need to sense and respond adequately to environmental stimuli. Metabolites provide information on systemic changes, for example during inflammation. We show that depletion of the amino acid arginine during inflammation reduced nuclear levels of its cognate aminoacyl tRNA synthetase, Arginyl-tRNA synthetase (ArgRS). In the nucleus, ArgRS interacted with serine/arginine repetitive matrix protein 2 (SRRM2), a spliceosomal protein and nuclear speckle organizer, in distinct condensate-like structures. ArgRS’ presence in the nucleus impeded SRRM2 mobility. Differences in SRRM2 speckle trafficking were directly reflected in alternative mRNA splicing: splice site usage was regulated in the opposite direction by ArgRS as by SRRM2, suggesting sequestration of SRRM2 by ArgRS as a regulatory mechanism. ArgRS- and SRRM2-dependent mRNA processing changes cumulated in altered metabolism and peptide presentation to immune cells. Our findings indicate a general mechanism in which ArgRS and SRRM2 act together as upstream regulators during the physiological response to inflammatory injury.
HostingRepository	PRIDE
AnnounceDate	2025-06-12
AnnouncementXML	Submission_2025-06-12_08:40:23.251.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027531
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Haissi Cui
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-25 14:31:41	ID requested
⏵ 1	2025-06-12 08:40:23	announced

10.1038/S41556-023-01118-8;

10.6019/PXD027531;

submitter keyword: inflammation, MHCI, mRNA splicing

Paul Schimmel
contact affiliation	Scripps Laboratories for tRNA Synthetase Research The Scripps Research Institute 10550 North Torrey Pines Road, BCC379 La Jolla, CA 92037
contact email	schimmel@scripps.edu
lab head
Haissi Cui
contact affiliation	University of Toronto
contact email	hacui@scripps.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027531
     1. Label: PRIDE project
     2. Name: MHCI peptidome of HepG2 with ArgRS or SRRM2 knock down