PXD027527-1

PXD027527 is an original dataset announced via ProteomeXchange.

Title	Genomic studies controvert the existence of the CUX1 p75 isoform
Description	CUX1, a homeodomain-containing transcription factor, is recurrently deleted or mutated in multiple tumor types. In myeloid neoplasms, CUX1 deletion or mutation carries a poor prognosis. We have previously established that CUX1 functions as a tumor suppressor in hematopoietic cells across multiple organisms. Others, however, have described oncogenic functions of CUX1 in solid tumors, often attributed to truncated CUX1 isoforms, p75 and p110. Given the clinical relevance, it is imperative to clarify these discrepant activities. Herein, we sought to determine the CUX1 isoforms expressed in hematopoietic cells, and find that they express the full-length p200 isoform. Through the course of this analysis, we found no evidence of the p75 alternative transcript in any cell type examined. Using an array of orthogonal approaches, including biochemistry, proteomics, CRISPR/Cas9 genomic editing, and analysis of functional genomics datasets across a spectrum of normal and malignant tissue types, we found no data to support the existence of the CUX1 p75 isoform generated by an alternative transcriptional start site. Based on these results, prior studies of p75 require reevaluation, including the interpretation of oncogenic roles attributed to CUX1.
HostingRepository	PRIDE
AnnounceDate	2022-02-17
AnnouncementXML	Submission_2022-02-17_05:38:28.229.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Donald Wolfgeher
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-25 13:55:06	ID requested
⏵ 1	2022-02-17 05:38:29	announced

Krishnan M, Senagolage MD, Baeten JT, Wolfgeher DJ, Khan S, Kron SJ, McNerney ME, Genomic studies controvert the existence of the CUX1 p75 isoform. Sci Rep, 12(1):151(2022) [pubmed]

submitter keyword: Q-Exactive, Human, KG-1 cell line, Cux1

Dr. Megan E. McNerney, MD, PhD
contact affiliation	The University of Chicago Medicine Comprehensive Cancer Center, University of Chicago, Chicago, IL USA Department of Pathology Department of Pediatrics Committee on Cancer Biology
contact email	Megan.McNerney@uchospitals.edu
lab head
Donald Wolfgeher
contact affiliation	University of Chicago
contact email	donw@uchicago.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027527
     1. Label: PRIDE project
     2. Name: Genomic studies controvert the existence of the CUX1 p75 isoform