PXD027520-2

PXD027520 is an original dataset announced via ProteomeXchange.

Title	The Angiostrongylus vasorum excretory/secretory proteome contains putative modulators of the host coagulation
Description	Angiostrongylus vasorum is a cardiopulmonary nematode of canids and is, among others associated with bleeding disorders in dogs. The pathogenesis of coagulopathies remains unclear. We performed a deep proteomic characterization of sex specific A. vasorum excretory/secretory proteins (ESP) and of cuticular surface proteins, and evaluated the effect of ESP on host coagulation and fibrinolysis in vitro. Proteins were quantified by liquid chromatography coupled to mass spectrometry and functionally characterized through gene ontology and pathway enrichment analysis. In total, we identified 1069 ESP (944 from female and 959 from male specimens) and 1195 surface proteins (705 and 1135, respectively). Among these, we identified putative modulators of host coagulation, e.g., von Willebrand factor type D domain protein orthologues as well as several proteases, including serine type proteases, protease inhibitors and proteasome subunits. The effect of ESP on dog coagulation and fibrinolysis was evaluated on canine endothelial cells and by rotational thromboelastometry (ROTEM). After stimulation with ESP, tissue factor and serpin E1 transcript expression increased. ROTEM revealed minimal interaction of ESP with dog blood and did not influence the onset of fibrinolysis. We thus conclude that Angiostrongylus vasorum ESP and surface proteins do not act alone in the induction of bleeding in dogs. The interaction with the host’s vascular haemostasis is limited. It is likely that coagulopathies in A. vasorum infected dogs are the result of a multifactorial response of the host to this parasitic infection.
HostingRepository	PRIDE
AnnounceDate	2024-10-22
AnnouncementXML	Submission_2024-10-22_05:30:06.339.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Nina Gillis
SpeciesList	scientific name: Angiostrongylus vasorum; NCBI TaxID: 321387;
ModificationList	acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-23 08:03:47	ID requested
1	2021-11-03 06:26:49	announced
⏵ 2	2024-10-22 05:30:06	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: Angiostrongylus vasorum, excretory/secretory, cuticular surface

Manuela Schnyder
contact affiliation	Veterinary Parasitology Institute of Parasitology University of Zurich Switzerland
contact email	Manuela.Schnyder@uzh.ch
lab head
Nina Gillis
contact affiliation	Institute of Parasitology, University of Zurich
contact email	nina.gillis@uzh.ch
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027520
     1. Label: PRIDE project
     2. Name: The Angiostrongylus vasorum excretory/secretory proteome contains putative modulators of the host coagulation