PXD027452-1

PXD027452 is an original dataset announced via ProteomeXchange.

Title	Annexin A1 is a polarity cue that directs planar mitotic spindle orientation during mammalian epithelial morphogenesis
Description	Oriented cell divisions are critical for the formation and maintenance of structured epithelia. Proper mitotic spindle orientation relies on polarised anchoring of force generators to the cell cortex by the evolutionarily conserved Gαi-LGN-NuMA complex. However, the polarity cues that control cortical patterning of this ternary complex remain largely unknown in mammalian epithelia. Here we identify the membrane-associated protein Annexin A1 (ANXA1) as a novel interactor of LGN in mammary epithelial cells. ANXA1 acts independently of Gαi to instruct the accumulation of LGN and NuMA at the lateral cortex to ensure cortical anchoring of Dynein-Dynactin and astral microtubules and thereby planar alignment of the mitotic spindle. Loss of ANXA1 randomises mitotic spindle orientation, which in turn disrupts epithelial architecture and lumen formation in three-dimensional (3D) primary mammary organoids. Our findings establish ANXA1 as an upstream cortical cue that regulates LGN to direct planar cell divisions during mammalian epithelial morphogenesis.
HostingRepository	PRIDE
AnnounceDate	2022-12-02
AnnouncementXML	Submission_2022-12-02_03:52:06.238.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	PaulSkipp
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-20 22:26:38	ID requested
⏵ 1	2022-12-02 03:52:06	announced

Dataset with its publication pending

submitter keyword: mammary,Proteomics, Annexin A1

SalahElias
contact affiliation	Biological Sciences University of Southampton
contact email	s.k.elias@soton.ac.uk
lab head
PaulSkipp
contact affiliation	Centre for Proteomic Research, University of Southampton
contact email	pjss@soton.ac.uk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027452
     1. Label: PRIDE project
     2. Name: Annexin A1 is a polarity cue that directs planar mitotic spindle orientation during mammalian epithelial morphogenesis