PXD027451-2

PXD027451 is an original dataset announced via ProteomeXchange.

Title	Bassoon contributes to tau-seed neurotoxicity and propagation.
Description	Tau aggregates are critical pathological features of Alzheimer’s disease (AD) and other tauopathies. Growing evidence suggests that soluble tau aggregates trigger neurodegenerative phenotypes. However, the nature of the tau species and interactors involved in its aggregation and spreading remains unclear. By using size exclusion chromatography, mass spectrometry, and bioinformatic analysis, we identified Bassoon protein (BSN) as a significant tau interactor in PS19 mice, as well as in human AD and PSP cases. We also found that overexpression of BSN triggers the aggregation of tau and increase the tau seeding activity in vitro, and also exacerbates the degenerative phenotype in a Fly model for tauopathy. Knockdown of BSN significantly reduced tau spreading in PS19 mouse brains and destabilized the tau aggregates, leading to a reduction in the tau pathology in this model. Furthermore, BSN downregulation was able to restore the neurodegenerative phenotype in PS19 mice, observed in electrophysiology and behavioral tests. Our results identify BSN as a key interactor of tau spread and aggregation in the brain, and therefore a potential target for the treatment of diseases that involve tau spread and aggregation.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:38:52.605.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027451
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Aruna Wijeratne
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; phosphorylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-20 22:25:29	ID requested
1	2023-03-11 00:02:54	announced
⏵ 2	2023-11-14 08:39:01	announced	2023-11-14: Updated project metadata.

Martinez P, Patel H, You Y, Jury N, Perkins A, Lee-Gosselin A, Taylor X, You Y, Viana Di Prisco G, Huang X, Dutta S, Wijeratne AB, Redding-Ochoa J, Shahid SS, Codocedo JF, Min S, Landreth GE, Mosley AL, Wu YC, McKinzie DL, Rochet JC, Zhang J, Atwood BK, Troncoso J, Lasagna-Reeves CA, Bassoon contributes to tau-seed propagation and neurotoxicity. Nat Neurosci, 25(12):1597-1607(2022) [pubmed]

10.6019/PXD027451;

submitter keyword: tauopathy, PSP, Bassoon., brain, AD, tau, neurotoxicity,LC-MSMS, aggregation, propagation

Cristian Lasagna-Reeves
contact affiliation	Department of Anatomy Cell Biology & Physiology Indiana University School of Medicine. Neurosciences Research Building 320 W. 15th Street, NB-214G Indianapolis, IN 46202
contact email	clasagna@iu.edu
lab head
Aruna Wijeratne
contact affiliation	Indiana University
contact email	arwije@iu.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/03/PXD027451

PRIDE project URI

• PRIDE
  1. PXD027451
     1. Label: PRIDE project
     2. Name: Bassoon contributes to tau-seed neurotoxicity and propagation.