PXD027443-1

PXD027443 is an original dataset announced via ProteomeXchange.

Title	Hyperosmolality in CHO Culture: Effects on Proteome
Description	Chinese hamster ovary cells (CHO) were exposed to highly concentrated feed solution during a fed-batch cultivation which causes an unphysiological osmolality increase (>300 mOsm/kg) affecting cell physiology, morphology and proteome. To get a deeper insight into underlying molecular mechanisms involved in cellular hyperosmolality response, we performed a comparative quantitative label-free proteome study of hyperosmolality-exposed vs. control CHO cells by nanoLC-ESI-MS measurement approach (orbitrap-MS). Our observations correlate well with the data from recent CHO-based fluxome and transcriptome studies and expose hitherto unknown targets involved in response to hyperosmotic pressure in mammalian cells.
HostingRepository	PRIDE
AnnounceDate	2022-05-20
AnnouncementXML	Submission_2022-05-20_02:58:39.990.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Louise Schelletter
SpeciesList	scientific name: Cricetulus griseus; NCBI TaxID: 10029;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-20 14:53:38	ID requested
⏵ 1	2022-05-20 02:58:40	announced

submitter keyword: CHO, fed-batch, hyperosmolality, cell size, nLC-MS LFQ proteomics, Q Exactive Plus

Prof. Thomas Noll
contact affiliation	Cell culture technology, Technical Department, Bielefeld University, Bielefeld, Germany
contact email	thomas.noll@uni-bielefeld.de
lab head
Louise Schelletter
contact affiliation	Bielefeld University
contact email	l.brachtvogel@uni-bielefeld.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027443
     1. Label: PRIDE project
     2. Name: Hyperosmolality in CHO Culture: Effects on Proteome