PXD027434 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cloning and Decoding of Chimpanzee Cytomegalovirus - A Resource for Comparative Functional Genomics |
Description | The chimpanzee cytomegalovirus (CCMV) is the closest relative of human CMV (HCMV). Because of the high conservation between these two species and the ability of human cells to fully support CCMV replication, CCMV holds great potential as a model system for HCMV. To make the CCMV genome available for precise and rapid gene manipulation techniques, we captured the genomic DNA of CCMV strain Heberling as a bacterial artificial chromosome(BAC). Selected BAC clones were reconstituted to infectious viruses, growing to similar high titers as parental CCMV. DNA sequencing confirmed the integrity of our clones and led to the identification of two polymorphic loci within the CCMV genome. To re-evaluate the CCMV coding potential, we analyzed the transcriptome and proteome of infected cells and identified several novel ORFs, splice variants, and regulatory RNAs. We further characterized the dynamics of viral gene expression and found that in strong parallel to HCMV, CCMV proteins cluster into five distinct temporal classes. In addition, our datasets revealed that the host response to CCMV infection and the de-regulation of cellular pathways are in line with known hallmarks of HCMV infection. In a first functional experiment, we investigated a proposed frameshift mutation in UL128 that was suspected to restrict CCMV’s cell tropism. In fact, repair of this frameshift re-established productive CCMV infection in endothelial and epithelial cells, expanding the options of CCMV as an infection model. Thus, BAC-cloned CCMV can serve as a powerful tool for systematic approaches in comparative functional genomics, exploiting the close phylogenetic relationship between CCMV and HCMV. |
HostingRepository | PRIDE |
AnnounceDate | 2021-12-20 |
AnnouncementXML | Submission_2021-12-20_08:24:56.246.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Boris Bogdanow |
SpeciesList | scientific name: Panine betaherpesvirus 2; NCBI TaxID: 188763; scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | acetylated residue; monohydroxylated residue; deamidated residue |
Instrument | Orbitrap Fusion |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-20 04:56:18 | ID requested | |
⏵ 1 | 2021-12-20 08:24:56 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Cytomegalovirus, Chimpanzee, Decoding, Evolution |
Contact List
Fan Liu |
contact affiliation | Department of Structural Biology, Leibniz-Forschungsinstitut für Molekulare Pharmakologie (FMP), Robert-Rössle-Straße 10, 13125 Berlin, Germany |
contact email | Fliu@fmp-berlin.de |
lab head | |
Boris Bogdanow |
contact affiliation | MDC Berlin |
contact email | boris.bogdanow@mdc-berlin.de |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027434
- Label: PRIDE project
- Name: Cloning and Decoding of Chimpanzee Cytomegalovirus - A Resource for Comparative Functional Genomics