PXD027387-1

PXD027387 is an original dataset announced via ProteomeXchange.

Title	Conserved exchange of paralog proteins during neuronal differentiation
Description	Gene duplication enables the emergence of new functions by lowering the general evolutionary pressure. Previous studies have highlighted the role of specific paralog genes during cell differentiation, e.g., in chromatin remodeling complexes. It remains unexplored whether similar mechanisms extend to other biological functions and whether the regulation of paralog genes is conserved across species. Here, we analyze the expression of paralogs across human tissues, during development and neuronal differentiation in fish, rodents and humans. While ~80% of paralog genes are co-regulated, a subset of paralogs shows divergent expression profiles, contributing to variability of protein complexes. We identify 78 substitutions of paralog pairs that occur during neuronal differentiation and are conserved across species. Among these, we highlight a substitution between the paralogs Sec23a and Sec23b subunits of the COPII complex. Altering the ratio between these two genes via RNAi-mediated knockdown is sufficient to influence the differentiation of immature neuron. We propose that remodeling of the vesicular transport system via paralog substitutions is an evolutionary conserved mechanism enabling neuronal differentiation.
HostingRepository	PRIDE
AnnounceDate	2022-03-07
AnnouncementXML	Submission_2022-03-07_10:24:18.073.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Emilio Cirri
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	monohydroxylated residue; acetylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Exploris 480

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-18 23:36:54	ID requested
⏵ 1	2022-03-07 10:24:18	announced
2	2024-10-22 05:35:18	announced	2024-10-22: Updated project metadata.

Dataset with its publication pending

submitter keyword: neuronal differentiation, paralog, development, protein complex, transport, proteome

Alessandro Ori
contact affiliation	Leibniz Institute on Aging - Fritz Lipmann Institute (FLI) Beutenbergstraße 11 07745 Jena, Germany
contact email	Alessandro.Ori@leibniz-fli.de
lab head
Emilio Cirri
contact affiliation	Leibniz Institute on Ageing Fritz Lipmann Institute Jena
contact email	emilio.cirri@fli-leibniz.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027387
     1. Label: PRIDE project
     2. Name: Conserved exchange of paralog proteins during neuronal differentiation