PXD027378-2

PXD027378 is an original dataset announced via ProteomeXchange.

Title	Analysis of phosphorylation sites of ZmRR1 phosphorylated by ZmMAPK8
Description	To identify the phosphorylation site of ZmRR1 by ZmMPK8 in vitro, 10 µg GST-ZmRR1 and 1 µg His-ZmMPK8 purified proteins were incubated in 20 µl of protein kinase reaction buffer containing 20 mM MgCl2, 50 mM Tris-HCl pH 7.5, 1 mM DTT, and 50 µM ATP, at 30°C for 30 min. The reaction products were reduced by DTT and alkylated by IAM (Iodoacetamide), followed by digestion with trypsin (pH 8.5) at 37°C for 12 h. The results were analyzed by LC-MS/MS
HostingRepository	iProX
AnnounceDate	2021-07-16
AnnouncementXML	Submission_2022-12-19_18:08:17.789.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Rong Zeng
SpeciesList	scientific name: Zea mays; NCBI TaxID: 4577;
ModificationList	phosphorylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-18 20:48:10	ID requested
1	2021-07-18 20:48:38	announced
⏵ 2	2022-12-19 18:08:18	announced	2022-12-20: Update information publication.

Zeng R, Li Z, Shi Y, Fu D, Yin P, Cheng J, Jiang C, Yang S, Natural variation in a type-A response regulator confers maize chilling tolerance. Nat Commun, 12(1):4713(2021) [pubmed]

submitter keyword: ZmRR1,ZmMAPK8,phospho-sites

Shuhua Yang
contact affiliation	China Agricultural University
contact email	yangshuhua@cau.edu.cn
lab head
Rong Zeng
contact affiliation	China Agricultural University
contact email	1603998769@qq.com
dataset submitter

iProX dataset URI