PXD027373-1
PXD027373 is an original dataset announced via ProteomeXchange.
Dataset Summary
Title | Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes |
Description | The biogenesis of mammalian autophagosomes remains to be fully defined. Here we used cellular and in vitro membrane fusion analyses to show that autophagosomes are formed from a hitherto unappreciated hybrid membrane compartment. The autophagic precursors emerge through fusion of FIP200 vesicles, derived from the cis-Golgi, with endosomally-derived ATG16L1 membranes to generate a hybrid pre-autophagosomal structure, HyPAS. HyPAS biogenesis depends on a previously unrecognized machinery centered upon STX17 interactors, the calcium pump SERCA2, extended synaptotagmin E-SYT2, and SIGMAR1. This apparatus controls HyPAS biogenesis and defines the mammalian autophagosomal precursor membranes. HyPAS can be modulated by pharmacological agents whereas its formation is inhibited upon SARS-CoV-2 infection or by expression of SARS-CoV-2 nsp6. These findings reveal the origin of mammalian autophagosomal membranes that emerge via convergence of secretory and endosomal pathways, and that this process is targeted by microbial factors such as coronaviral membrane modulating proteins. |
HostingRepository | MassIVE |
AnnounceDate | 2021-11-09 |
AnnouncementXML | Submission_2021-11-09_11:38:31.628.xml |
DigitalObjectIdentifier | |
ReviewLevel | Non peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Brett Phinney |
SpeciesList | scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606; |
ModificationList | Oxidation; Carbamidomethyl |
Instrument | Orbitrap Exploris 480 |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
---|---|---|---|
0 | 2021-07-16 15:40:12 | ID requested | |
⏵ 1 | 2021-11-09 11:38:31 | announced |
Publication List
no publication |
Keyword List
submitter keyword: autophagosomes, HyPAS |
Contact List
Vojo Deretic | |
---|---|
contact affiliation | University of New Mexico Health Sciences Center |
contact email | vderetic@salud.unm.edu |
lab head | |
Brett Phinney | |
contact affiliation | UC Davis |
contact email | brettsp@ucdavis.edu |
dataset submitter |
Full Dataset Link List
MassIVE dataset URI |
Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087840/ |