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PXD027373-1

PXD027373 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleMammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes
DescriptionThe biogenesis of mammalian autophagosomes remains to be fully defined. Here we used cellular and in vitro membrane fusion analyses to show that autophagosomes are formed from a hitherto unappreciated hybrid membrane compartment. The autophagic precursors emerge through fusion of FIP200 vesicles, derived from the cis-Golgi, with endosomally-derived ATG16L1 membranes to generate a hybrid pre-autophagosomal structure, HyPAS. HyPAS biogenesis depends on a previously unrecognized machinery centered upon STX17 interactors, the calcium pump SERCA2, extended synaptotagmin E-SYT2, and SIGMAR1. This apparatus controls HyPAS biogenesis and defines the mammalian autophagosomal precursor membranes. HyPAS can be modulated by pharmacological agents whereas its formation is inhibited upon SARS-CoV-2 infection or by expression of SARS-CoV-2 nsp6. These findings reveal the origin of mammalian autophagosomal membranes that emerge via convergence of secretory and endosomal pathways, and that this process is targeted by microbial factors such as coronaviral membrane modulating proteins.
HostingRepositoryMassIVE
AnnounceDate2021-11-09
AnnouncementXMLSubmission_2021-11-09_11:38:31.628.xml
DigitalObjectIdentifier
ReviewLevelNon peer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterBrett Phinney
SpeciesList scientific name: Homo sapiens; common name: human; NCBI TaxID: 9606;
ModificationListOxidation; Carbamidomethyl
InstrumentOrbitrap Exploris 480
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-16 15:40:12ID requested
12021-11-09 11:38:31announced
Publication List
no publication
Keyword List
submitter keyword: autophagosomes, HyPAS
Contact List
Vojo Deretic
contact affiliationUniversity of New Mexico Health Sciences Center
contact emailvderetic@salud.unm.edu
lab head
Brett Phinney
contact affiliationUC Davis
contact emailbrettsp@ucdavis.edu
dataset submitter
Full Dataset Link List
MassIVE dataset URI
Dataset FTP location
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