PXD027371-1

PXD027371 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Trypanosoma cruzi proteins identified in pull-down assays aimed to characterize RNA proteins interacting with LYT1 mRNAs
Description	This project was aimed to identify proteins interacting with the untranslated regions (UTRs) of the mRNAs transcribed from the Trypanosoma cruzi LYT1 gene. LYT1 protein is a T. cruzi virulence factor, which is expressed as two isoforms: kLYT1 and mLYT1. The proteomic data associated with this project derive from three biological replicas of pull-down assays in which particular regions of the LYT1 mRNAs (5 UTR kLYT1, 5 UTR mLYT1, and 3 UTRs types I and II) were used as baits. These RNA baits were incubated with crude extracts from either epimastigotes or trypomastigotes of the T. cruzi 058PUJ (DTU I) strain. After washing out of non-bound proteins, the remaining proteins were analyzed by liquid chromatography coupled to mass spectrometry (LC/MS). This project contains the LC/MS raw spectra generated in these assays.
HostingRepository	MassIVE
AnnounceDate	2022-01-31
AnnouncementXML	Submission_2022-01-31_13:04:31.589.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	C Judith Puerta Bula
SpeciesList	scientific name: Trypanosoma cruzi; NCBI TaxID: 5693;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600+

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-16 07:50:38	ID requested
⏵ 1	2022-01-31 13:04:31	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: LC/MS, pull-down, RNA Binding Proteins, Trypanosoma cruzi, Untranslated regions

submitter keyword: LC/MS, pull-down, RNA Binding Proteins, Trypanosoma cruzi, Untranslated regions

Contact List

Concepcion Judith Puerta Bula
contact affiliation	Pontificia Universidad Javeriana
contact email	cpuerta@javeriana.edu.co
lab head
C Judith Puerta Bula
contact affiliation	Pontificia Universidad
contact email	cpuerta@javeriana.edu.co
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087835/