PXD027353-1

PXD027353 is an original dataset announced via ProteomeXchange.

Title	Variability in the Glycosylation Patterns of gp120 Proteins from Different Human Immunodeficiency Virus Type 1 Isolates Expressed in Different Host Cells
Description	The mature HIV-1 envelope (Env) glycoprotein is composed of gp120, the exterior subunit, and gp41, the transmembrane subunit assembled as trimer by noncovalent interaction. There is a great body of literature to prove that gp120 binds to CD4 first, then to the co-receptor. We have recently demonstrated that gp120 cannot bind to the co-receptor without first interacting with CD4. Previous studies provided different glycomic maps for the HIV-1 gp120. Here, we build on previous work to report that the use of LC-MS/MS, in conjunction with hydrophilic interaction liquid chromatography (HILIC) enrichment to glycosylation sites, is associated with the assorted neutralizing or binding events of glycosylation targeted antibodies from different clades or strains. In this study, the microheterogeneity of the glycosylation from 4 different clades of gp120s is deeply investigated. Aberrant glycosylation patterns were detected on gp120 originated from different clades, viral sequences and host cells. The results of this study may help provide better understanding of the mechanism of how the glycans participate in antibody neutralizing process that target glycosylation sites.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_10:54:55.940.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Yifan Huang
SpeciesList	scientific name: Human immunodeficiency virus 1; NCBI TaxID: 11676;
ModificationList	acetylated residue; deaminated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-15 23:51:30	ID requested
⏵ 1	2024-10-07 10:54:56	announced

Dataset with its publication pending

submitter keyword: microheterogeneity, glycoproteomics,gp120, HIV isolates, glycopeptides, LC-MS/MS

Yehia Mechref
contact affiliation	Texas Tech University
contact email	yehia.mechref@ttu.edu
lab head
Yifan Huang
contact affiliation	Texas Tech University
contact email	yifan.huang@ttu.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027353
     1. Label: PRIDE project
     2. Name: Variability in the Glycosylation Patterns of gp120 Proteins from Different Human Immunodeficiency Virus Type 1 Isolates Expressed in Different Host Cells