PXD027338-1

PXD027338 is an original dataset announced via ProteomeXchange.

Title	Combined Metabolic and Chemical (CoMetChem) labeling using stable isotopes
Description	Histone acetylation is an important, reversible posttranslational protein modification and hallmark of epigenetic regulation. However, little is known about the dynamics of reversible hisotne acetylation, due to the lack of analytical methods that can capture site-specific acetylation and deacetylation reactions. We present a new approach that combines metabolic and chemical labeling (CoMetChem) using uniformly 13C-labeled glucose and stable isotope labeled acetic anhydride, which allows to quantify site-specific lysine acetylation dynamics in tryptic peptides using high-resolution mass spectrometry.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:41:33.321.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Anna-Sophia Egger
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	2xC(13),3x(2)H labeled N6-acetyl-L-lysine; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos; Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-15 09:29:50	ID requested
⏵ 1	2024-10-07 13:41:34	announced

van Pijkeren A, Dietze J, Brotons AS, Egger AS, Lijster T, Barcaru A, Hotze M, Kobler P, Dekker FJ, Horvatovich P, Melgert BN, Ziegler M, Thedieck K, Heiland I, Bischoff R, Kwiatkowski M, Combined Metabolic and Chemical (CoMetChem) Labeling Using Stable Isotopes-a Strategy to Reveal Site-Specific Histone Acetylation and Deacetylation Rates by LC-MS. Anal Chem, 93(38):12872-12880(2021) [pubmed]

10.1021/acs.analchem.1c01359;

submitter keyword: LC-MSMS,stable isotopes, acetylation, histones

Marcel Kwiatkowski
contact affiliation	Functional Proteo-Metabolomics Department of Biochemistry University of Innsbruck Austria
contact email	Marcel.Kwiatkowski@uibk.ac.at
lab head
Anna-Sophia Egger
contact affiliation	Institut für Biochemie Universität Innsbruck
contact email	anna-sophia.egger@uibk.ac.at
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD027338

PRIDE project URI

• PRIDE
  1. PXD027338
     1. Label: PRIDE project
     2. Name: Combined Metabolic and Chemical (CoMetChem) labeling using stable isotopes