PXD027318-1

PXD027318 is an original dataset announced via ProteomeXchange.

Title	Senescence Biomarker Discovery by LC-MS-based Proteomics
Description	Senescence is a permanent cell cycle arrest that occurs in response to cellular stress. Because senescent cells promote age-related disease, there has been considerable interest in defining the proteomic alterations in senescent cells. Because senescence differs greatly depending on cell type and senescence inducer, continued progress in the characterization of senescent cells is needed. Here, we analyzed primary human mammary epithelial cells (HMECs), a model system for aging, using mass spectrometry-based proteomics. By integrating data from replicative senescence, immortalization by telomerase reactivation, and drug-induced senescence, we identified a robust proteomic signature of HMEC senescence consisting of 77 upregulated and 36 downregulated proteins. This approach identified known biomarkers, such as downregulation of the nuclear lamina protein lamin-B1 (LMNB1), and novel upregulated proteins including the β-galactoside-binding protein galectin-7 (LGALS7). Gene ontology enrichment analysis demonstrated that senescent HMECs upregulated lysosomal proteins and downregulated RNA metabolic processes. We additionally integrated our proteomic signature of senescence with transcriptomic data from senescent HMECs to demonstrate that our proteomic signature can discriminate proliferating and senescent HMECs even at the transcriptional level. Taken together, our results demonstrate the power of proteomics to identify cell type-specific signatures of senescence and advance the understanding of senescence in primary HMECs.
HostingRepository	PRIDE
AnnounceDate	2021-09-28
AnnouncementXML	Submission_2021-09-28_09:17:11.451.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Alireza Delfarah
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-14 22:12:53	ID requested
⏵ 1	2021-09-28 09:17:11	announced

Dataset with its publication pending

submitter keyword: senescence, aging, proteomics, mammary epithelial cells

Nicholas Alexander Graham
contact affiliation	Mork Family Department of Chemical Engineering and Materials Science, University of Southern California, USA
contact email	nagraham@usc.edu
lab head
Alireza Delfarah
contact affiliation	University of Southern California
contact email	delfarah@usc.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027318
     1. Label: PRIDE project
     2. Name: Senescence Biomarker Discovery by LC-MS-based Proteomics