PXD027314-2

PXD027314 is an original dataset announced via ProteomeXchange.

Title	Juvenile hormone membrane signaling phosphorylates Hsp83 and promotes Met nuclear import
Description	JH modulates phosphorylation modification of components of its intracellular signaling through putative membrane pathway. As a transcriptional factor, Met phosphorylation is contributed to its transcriptional activity, but whether phosphorylation modification affects Met nuclear import is unknown. Here, we report that phosphorylation sites are dispensable for NLS activity whether JH is present or not. Interestingly, binding affinity of Met and Hsp83 fails to increase by adding JH is resulted from the mutant of JH-regulated phosphorylation of Hsp83 at S219. JH also increase binding affinity of Hsp83 and 14-3-3 proteins independent on S219. 14-3-3 proteins negatively regulated JH activity through sequestrating Met in the cytoplasm that dependent on Hsp83, which acts as a bridge between Met/Gce and 14-3-3 proteins. These results suggest that JH membrane pathway phosphorylates either Met or Hsp83 to promote JH intracellular signaling, meanwhile, a negative feedback loop forms between the 14-3-3 proteins and Hsp83.
HostingRepository	iProX
AnnounceDate	2021-07-15
AnnouncementXML	Submission_2022-12-19_18:07:26.682.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Suning Liu
SpeciesList	scientific name: Drosophila melanogaster; NCBI TaxID: 7227;
ModificationList	phosphorylated residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-14 20:13:28	ID requested
1	2021-07-14 20:14:10	announced
⏵ 2	2022-12-19 18:07:27	announced	2022-12-20: Update information publication.

Gao Y, Chen N, Zhang X, Li EY, Luo W, Zhang J, Zhang W, Li S, Wang J, Liu S, Juvenile Hormone Membrane Signaling Enhances its Intracellular Signaling Through Phosphorylation of Met and Hsp83. Front Physiol, 13():872889(2022) [pubmed]

submitter keyword: juvenile hormone, Hsp83, Met

Sheng Li
contact affiliation	South China Normal University
contact email	lisheng@scnu.edu.cn
lab head
Suning Liu
contact affiliation	South China Normal University
contact email	liusuning@m.scnu.edu.cn
dataset submitter

iProX dataset URI