PXD027265-1

PXD027265 is an original dataset announced via ProteomeXchange.

Title	A universal peptide matrix interactomics approach to disclose motif dependent protein binding
Description	Protein-protein interactions (PPIs) mediated by intrinsically disordered regions (IDRs) are often based on short linear motifs (SLiM). SLiMs are implicated in signal transduction and gene regulation, yet remain technically laborious and notoriously challenging to study. Here, we present an optimized method for a PRotein Interaction Screen on a peptide MAtrix (PRISMA) in combination with quantitative mass spectrometry. The protocol was benchmarked with previously described SLiM based PPIs using peptides derived from EGFR, SOS1, GLUT1 and CEBPB and extended to map binding partners of kinase activation loops. The detailed protocol provides practical considerations for setting up a PRISMA screen and subsequently implementing PRISMA on a liquid handling robotic platform as a cost effective high-throughput method. Optimized PRISMA can be universally applied to systematically study SLiM based interactions and associated post translational modifications (PTMs) or mutations to advance our understanding of the largely uncharacterized interactomes of intrinsically disordered protein regions.
HostingRepository	PRIDE
AnnounceDate	2021-08-11
AnnouncementXML	Submission_2021-08-10_23:46:39.364.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Evelyn Ramberger
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	iodoacetamide derivatized residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-13 05:15:50	ID requested
⏵ 1	2021-08-10 23:46:39	announced

Dataset with its publication pending

submitter keyword: peptide interactomics, PTM, PRISMA

Philipp Mertins
contact affiliation	Max Delbrück Center for Molecular Medicine in the Helmholtz Association, Berlin, Germany
contact email	Philipp.Mertins@mdc-berlin.de
lab head
Evelyn Ramberger
contact affiliation	Max Delbrück Center for Molecular Medicine, Berlin
contact email	evelyn.ramberger@mdc-berlin.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027265
     1. Label: PRIDE project
     2. Name: A universal peptide matrix interactomics approach to disclose motif dependent protein binding