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PXD027229-1

PXD027229 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleCharacterisation of the free and membrane-associated fractions of the thylakoid lumen proteome in Arabidopsis thaliana
DescriptionIn plants chloroplasts, the lumen of the thylakoid membrane houses proteins that are vital for photosynthetic electron transport, including water-splitting at photosystem (PS) II and shuttling of electrons from cytochrome b6f to PSI. Other lumen proteins maintain photosynthetic activity through biogenesis and turnover of PSII complexes. Although all lumen proteins are soluble, these known details have highlighted interactions of some lumen proteins with thylakoid membranes or thylakoid-intrinsic proteins. Meanwhile, the functional details of most lumen proteins, as well as their distribution between the soluble and membrane-associated lumen fractions, remain unknown. The current study isolated the soluble free lumen (FL) and membrane-associated lumen (MAL) fractions from Arabidopsis thaliana, and used gel- and mass spectrometry-based proteomics methods to analyse the contents of each proteome. These results identified lumenal proteins, and clearly distinguished the difference between the FL and MAL proteomes. The most abundant proteins in the FL fraction were involved in PSII assembly and repair, while the MAL proteome was enriched in proteins that support of the oxygen-evolving complex (OEC). Novel proteins, including a new PsbP domain-containing isoform, as well as several novel post-translational modifications and N-termini, are reported, and bi-dimensional separation of the lumen proteome identified several potential multi-protein oligomers in the thylakoid lumen.
HostingRepositoryPRIDE
AnnounceDate2021-07-30
AnnouncementXMLSubmission_2021-07-30_05:02:15.922.xml
DigitalObjectIdentifierhttps://dx.doi.org/10.6019/PXD027229
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportSupported dataset by repository
PrimarySubmitterAndrea Trotta
SpeciesList scientific name: Arabidopsis thaliana (Mouse-ear cress); NCBI TaxID: 3702;
ModificationListphosphorylated residue; acetylated residue; monohydroxylated residue; iodoacetamide derivatized residue
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-07-12 01:42:02ID requested
12021-07-30 05:02:16announced
Publication List
Dataset with its publication pending
Keyword List
submitter keyword: Arabidopsis thaliana, photosynthesis, chloroplast, thylakoid lumen, post-translational modification
Contact List
Eva-Mari Aro
contact affiliationMolecular Plant Biology, University of Turku, Finland
contact emailevaaro@utu.fi
lab head
Andrea Trotta
contact affiliationInstitute of Biosciences and Bioresources, National Research Council of Italy, Sesto Fiorentino (FI)
contact emailandrea.trotta@utu.fi
dataset submitter
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Dataset FTP location
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