PXD027220-1

PXD027220 is an original dataset announced via ProteomeXchange.

Title	Proteomic Analysis of Mkrn3 WT vs KO mouse brain
Description	Mutations in the E3 ubiquitin ligase Mkrn3 are associated with precocious puberty in humans. In order to determine the targets of Mkrn3, we performed a TMT-based proteomic analysis of Mkrn3 WT vs KO mouse brains.
HostingRepository	PRIDE
AnnounceDate	2023-07-20
AnnouncementXML	Submission_2023-07-20_10:46:24.392.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	BrandonGassaway
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	TMT6plex-126 reporter+balance reagent acylated residue; monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	Orbitrap Fusion

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-11 22:34:41	ID requested
⏵ 1	2023-07-20 10:46:25	announced
2	2023-11-14 09:02:44	announced	2023-11-14: Updated project metadata.
3	2024-10-22 05:54:51	announced	2024-10-22: Updated project metadata.

Naul, é L, Mancini A, Pereira SA, Gassaway BM, Lydeard JR, Magnotto JC, Kim HK, Liang J, Matos C, Gygi SP, Merkle FT, Carroll RS, Abreu AP, Kaiser UB, MKRN3 inhibits puberty onset via interaction with IGF2BP1 and regulation of hypothalamic plasticity. JCI Insight, 8(8):(2023) [pubmed]

submitter keyword: Mouse, Brain, TMT, E3 ubiquitin ligase, Precocious Puberty

StevenGygi
contact affiliation	Harvard University Medical School, Department of Cell Biology
contact email	steven_gygi@hms.harvard.edu
lab head
BrandonGassaway
contact affiliation	Harvard Medical School
contact email	brandon_gassaway@hms.harvard.edu
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2023/07/PXD027220

PRIDE project URI

• PRIDE
  1. PXD027220
     1. Label: PRIDE project
     2. Name: Proteomic Analysis of Mkrn3 WT vs KO mouse brain