PXD027193 is an
original dataset announced via ProteomeXchange.
Dataset Summary
| Title | Characterization of waxy starches from CRISPR-Cas9 edited potato plants, the loss of GBSS1 influences the phosphate con-tent inside the starch granule |
| Description | The granule-bound starch synthase is responsible for amylose biosynthesis. This enzyme is ac-tive when physically associated with starch granules where it represents up to 95% of starch-bound proteins. gbss1 mutants of Solanum tuberosum, cv Desiree were recently generated by CRIPR/Cas9-driven gene edition. Here, we investigated the impact of the modulation of GBSS1 contents on the starch-bound proteome and the resulting amylopectin structure. The total protein content in knock-out mutants was decreased by 50 %, which indicate a compensation by the other proteins since GBSS1 represent 85 % of the total protein content. This compensation was mainly due to the increase of starch-bound SS2 (starch synthase 2) and GWD (glucan water dikinase). These changes were accompanied by an increase of the phosphate content. Taken to-gether, these data reveal that GBSS1 loss is compensated by the association of other starch met-abolic enzymes with starch granules, with a strong increase of GWD, leading to modified amy-lopectin structure. |
| HostingRepository | PRIDE |
| AnnounceDate | 2026-01-26 |
| AnnouncementXML | Submission_2026-01-26_07:29:13.819.xml |
| DigitalObjectIdentifier | |
| ReviewLevel | Peer-reviewed dataset |
| DatasetOrigin | Original dataset |
| RepositorySupport | Unsupported dataset by repository |
| PrimarySubmitter | BRAY FABRICE |
| SpeciesList | scientific name: Solanum tuberosum (Potato); NCBI TaxID: NEWT:4113; |
| ModificationList | carbamoylated residue; acetylated residue; monohydroxylated residue; deamidated residue |
| Instrument | Q Exactive Plus |
Dataset History
| Revision | Datetime | Status | ChangeLog Entry |
|---|
| 0 | 2021-07-08 09:10:33 | ID requested | |
| ⏵ 1 | 2026-01-26 07:29:14 | announced | |
Publication List
| 10.1016/j.ijbiomac.2026.150329; |
| Helle S, Sevestre F, Veillet F, Bray F, Flament S, Derbass W, Chauvin L, Rolando C, D'Hulst C, Chauvin JE, Szydlowski N, Loss of the granule-bound starch synthase protein reshapes the starch proteome and phosphorylation profile in potato tuber. Int J Biol Macromol, 343(Pt 2):150329(2026) [pubmed] |
Keyword List
| submitter keyword: Proteomics |
| Starch |
| GBSS1 |
| waxy |
| GWD |
| phosphorylation |
| chain length distribution |
| metabolism |
Contact List
| Nicolas Szydlowski |
|---|
| contact affiliation | Univ. Lille, CNRS, UMR8576 – UGSF – Unité de Glycobiologie Structurale et Fonctionnelle, F-59000 Lille, France Univ. Lille, CNRS, USR 3290 – MSAP – Miniaturisation pour la Synthèse l’Analyse et la Protéomique, F-59000 Lille, France |
| contact email | nicolas.szydlowski@univ-lille.fr |
| lab head | |
| BRAY FABRICE |
|---|
| contact affiliation | MSAP USR 3290 |
| contact email | fabrice.bray@univ-lille.fr |
| dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2026/01/PXD027193 |
| PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027193
- Label: PRIDE project
- Name: Characterization of waxy starches from CRISPR-Cas9 edited potato plants, the loss of GBSS1 influences the phosphate con-tent inside the starch granule
