PXD027163-1

PXD027163 is an original dataset announced via ProteomeXchange.

Title	The inflammation repressor TNIP1 is degraded by selective autophagy in a LIR-dependent manner upon TLR3 activation
Description	In the project « The inflammation repressor TNIP1 is degraded by selective autophagy in a LIR-dependent manner upon TLR3 activation » Jianwen Zhou, Nikoline Lander Rasmussen, Vyacheslav Akimov, Blagoy Blagoev, Trond Lamark, Terje Johansen and Jörn Dengjel performed MS-based proteomics to study the function of TNIP1. Three set of SILAC experiments were performed (classical double-triple labeling): (1) Global analysis of ubiquitination events using the UbiSite approach. Three biological replicates were performed using following SILAC labeling (1) Protein-protein interactions of TNIP1 performing HA-TNIP1 AP-MS analyses. Three biological replicates were performed using following SILAC labeling (3) Expression proteomics comparing WT and different TNIP1 ko clones. Six biological replicates were performed using following SILAC labeling
HostingRepository	PRIDE
AnnounceDate	2022-08-07
AnnouncementXML	Submission_2022-08-07_15:15:17.429.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Joern Dengjel
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	ubiquitinylated lysine
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-07 07:04:41	ID requested
⏵ 1	2022-08-07 15:15:17	announced

Dataset with its publication pending

submitter keyword: human, inflammation, LC-MSMS, SILAC

Joern Dengjel
contact affiliation	Dr. Jörn Dengjel Department of Biology, University of Fribourg Chemin du Musée 10, 1700 Fribourg, Switzerland T +41 26 300 8631 E joern.dengjel@unifr.ch
contact email	joern.dengjel@unifr.ch
lab head
Joern Dengjel
contact affiliation	University of Fribourg
contact email	joern.dengjel@medizin.uni-freiburg.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD027163

PRIDE project URI

• PRIDE
  1. PXD027163
     1. Label: PRIDE project
     2. Name: The inflammation repressor TNIP1 is degraded by selective autophagy in a LIR-dependent manner upon TLR3 activation