PXD027156 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Cysteamine inhibits glycine utilisation and disrupts virulence in Pseudomonas aeruginosa |
Description | Pseudomonas aeruginosa is a major opportunistic human pathogen which employs a myriad of virulence factors. In people with cystic fibrosis (CF) P. aeruginosa frequently colonises the lungs and becomes a chronic infection that evolves to become less virulent over time, but often adapts to favour persistence in the host with alginate-producing mucoid, slow-growing, and antibiotic resistant phenotypes emerging. Cysteamine is an endogenous aminothiol which has been shown to prevent biofilm formation, reduce phenazine production, and potentiate antibiotic activity against P. aeruginosa, and has been investigated in clinical trials as an adjunct therapy for pulmonary exacerbations of CF. Here we demonstrate (for the first time in a prokaryote) that cysteamine prevents glycine utilisation by P. aeruginosa in common with reported activity blocking the glycine cleavage system in human cells. Despite the inhibition of glycine metabolism, cysteamine inhibits hydrogen cyanide (HCN) production by P. aeruginosa, implicating interference in the regulation of virulence. Cysteamine impaired chemotaxis and reductions in pyocyanin and exopolysaccharide production in cysteamine-treated P. aeruginosa and reduced toxicity of secreted factors in a Galleria mellonella model. Thus, cysteamine has additional potent anti-virulence properties targeting P. aeruginosa, further supporting its therapeutic potential in CF and other infections. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-19 |
AnnouncementXML | Submission_2021-08-19_08:02:05.161.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Kevin Mclean |
SpeciesList | scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue; deamidated residue |
Instrument | Bruker Daltonics amaZon series |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-07 04:07:29 | ID requested | |
⏵ 1 | 2021-08-19 08:02:05 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Pseudomonas aeruginosa , Human |
Contact List
Kevin Mclean |
contact affiliation | Moredun Proteomics Facility Moredun Research Institute |
contact email | kevin.mclean@moredun.ac.uk |
lab head | |
Kevin Mclean |
contact affiliation | Moredun Resaerch INstitute |
contact email | kevin.mclean@moredun.ac.uk |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027156
- Label: PRIDE project
- Name: Cysteamine inhibits glycine utilisation and disrupts virulence in Pseudomonas aeruginosa