PXD027149-1

PXD027149 is an original dataset announced via ProteomeXchange.

Title	A Chemical Tool for Blue Light-Inducible Proximity Photo-crosslinking in Live Cells
Description	We developed a proximity photo-crosslinking method (Spotlight) with a 4-azido-N-ethyl-1,8-naphthalimide (AzNP) moiety that can be converted to reactive aryl nitrene species using ambient blue light-emitting diode light. Using an AzNP-conjugated HaloTag ligand (VL1), blue light-induced photo-crosslinked products of various HaloTag-conjugated proteins of interest were detected in subcellular spaces in live cells. Using Spotlight, we further identified the host interactome of SARS-CoV-2 nucleocapsid (N) protein, which is essential for viral genome assembly. Mass analysis of the VL1-crosslinked product of N-HaloTag in HEK293T cells showed that RNA-binding proteins in stress granules were exclusively enriched in the cross-linked samples. These results tell that our method can reveal the interactome of protein of interest within a short distance in live cells.
HostingRepository	PRIDE
AnnounceDate	2022-05-31
AnnouncementXML	Submission_2022-05-31_07:02:12.392.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD027149
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Hyun-Woo Rhee
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	carbamoylated residue; acetylated residue; monohydroxylated residue
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-07 02:17:00	ID requested
⏵ 1	2022-05-31 07:02:13	announced

Mishra PK, Kang MG, Lee H, Kim S, Choi S, Sharma N, Park CM, Ko J, Lee C, Seo JK, Rhee HW, A chemical tool for blue light-inducible proximity photo-crosslinking in live cells. Chem Sci, 13(4):955-966(2022) [pubmed]

submitter keyword: Proximity crosslinking / SARS-CoV-2 / Nucleocapsid

Hyun-Woo Rhee
contact affiliation	Department of Chemistry, Seoul National University, Seoul 08826, Korea
contact email	rheehw@snu.ac.kr
lab head
Hyun-Woo Rhee
contact affiliation	Department of Chemistry, Seoul National University, Seoul 08826, Korea
contact email	rheehw@snu.ac.kr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027149
     1. Label: PRIDE project
     2. Name: A Chemical Tool for Blue Light-Inducible Proximity Photo-crosslinking in Live Cells