PXD027132 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Casein phoshopeptides identification by partial-, double-dephosphorylation and endoGluC digestion |
Description | The aim of the present study was the development of a relatively simple method without enrichment step to detect, in a mixture of peptides and phosphopeptides, a maximum of CPPs, including multi-phosphorylated and large-size CPPs, by using high pressure liquid chromatography (HPLC), high-resolution mass spectrometry (MS) and bioinformatics. A commercial casein hydrolysate was used as model hydrolysate for this purpose. This approach consisted of use of double and partial dephosphorylations of CPPs as well as digestion by EndoGluC protease before peptidomics analysis. |
HostingRepository | PRIDE |
AnnounceDate | 2021-09-29 |
AnnouncementXML | Submission_2021-09-29_05:03:11.323.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Barbara Deracinois |
SpeciesList | scientific name: Bos taurus (Bovine); NCBI TaxID: 9913; |
ModificationList | phosphorylated residue; monohydroxylated residue |
Instrument | Synapt MS |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-06 08:58:26 | ID requested | |
⏵ 1 | 2021-09-29 05:03:12 | announced | |
Publication List
Deracinois B, Mat, é, os A, Romelard A, Boulier A, Auger J, Baniel A, Ravallec R, Flahaut C, Partial-, Double-Enzymatic Dephosphorylation and EndoGluC Hydrolysis as an Original Approach to Enhancing Identification of Casein Phosphopeptides (CPPs) by Mass Spectrometry. Foods, 10(9):(2021) [pubmed] |
Keyword List
submitter keyword: casein phosphopeptides, mass spectrometry, dephosphorylation, endoGluC digestion |
Contact List
Jean-Louis HILBERT |
contact affiliation | UMR Transfrontalière BioEcoAgro N° 1158,Univ. Lille, INRAE, Univ. Liège, UPJV, YNCREA, Univ. Artois, Univ. Littoral Côte d’Opale, ICV – Institut Charles Viollette, F-59000 Lille, France. |
contact email | jean-louis.hilbert@univ-lille.fr |
lab head | |
Barbara Deracinois |
contact affiliation | UMRT BioEcoAgro |
contact email | barbara.deracinois@univ-lille.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027132
- Label: PRIDE project
- Name: Casein phoshopeptides identification by partial-, double-dephosphorylation and endoGluC digestion