PXD027114-2

PXD027114 is an original dataset announced via ProteomeXchange.

Title	Integrative analysis from the transcriptome to proteome uncovers brassinosteroid-mediated regulation of cambium initiation and patterning in woody stems
Description	Wood formation involves sequential developmental events, requiring the coordination of multiple hormones. Brassinosteroids (BRs) play a key role in wood development, but little is known about the cellular and molecular processes that underlie wood formation in tree species. Here, we generated transgenic poplar lines editing one or several members of PdBRI1s, the orthologs of Arabidopsis vascular-enriched BR receptors, and showed how inhibition of BR signaling influences wood development at the proteome levels. Six Populus PdBRI1 genes form three gene pairs and each exhibits high expression in the basal stems. Simultaneous mutation of PdBRI1-1, 2, 3 and 6, the orthologs of Arabidopsis vascular-enriched BR receptors BRI1, BRL1 and BRL3, resulted in a severe defect in growth. Particularly, the stems of these mutant lines displayed a discontinuous ring of cambium and patterning defects in derived secondary vascular tissues. Abnormal formation of cambia within cortical parenchyma was also observed in the stem of pdbri1-1;2;3;6. Transgenic poplars editing PdBRI1-1 or PdBRI1-1;2;6 had phenotypic alterations in stem development at 4.5 months of growth, indicating functional redundancy among these PdBRI1 genes. An analysis of the proteome from pdbri1-1;2;3;6 stems revealed differential expression of a number of proteins associated with wood development and hormones.
HostingRepository	iProX
AnnounceDate	2021-07-06
AnnouncementXML	Submission_2023-07-03_20:42:49.367.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Guohua Chai
SpeciesList	scientific name: Populus hybrid cultivar; NCBI TaxID: 1243876;
ModificationList	TMT6plex reporter fragment
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-05 20:18:12	ID requested
1	2023-07-03 01:52:37	announced
⏵ 2	2023-07-03 20:42:50	announced	2023-07-04: Update publication information.

Wang C, Liu N, Geng Z, Ji M, Wang S, Zhuang Y, Wang D, He G, Zhao S, Zhou G, Chai G, Integrated transcriptome and proteome analysis reveals brassinosteroid-mediated regulation of cambium initiation and patterning in woody stem. Hortic Res, 9():(2022) [pubmed]

submitter keyword: Brassinosteroid, wood formation, multidimensional regulation, Populus

Gongke Zhou
contact affiliation	College of Resources and Environment, Qingdao Agricultural University
contact email	zhougk@qau.edu.cn
lab head
Guohua Chai
contact affiliation	College of Resources and Environment, Qingdao Agricultural University
contact email	chaigh@qau.edu.cn
dataset submitter

iProX dataset URI