PXD027110-1

PXD027110 is an original dataset announced via ProteomeXchange.

Title	Surfaceome proteomic of glioblastoma revealed potential targets for immunotherapy
Description	Glioblastoma (GBM) is the most common and devastating malignant brain tumor in adults. The mortality rate is very high despite different treatments. New therapeutic targets are therefore highly needed to improve patient care. Cell-surface proteins represent attractive targets due to their accessibility, their involvement in essential signaling pathways, and their dysregulated expression in cancer. Moreover, they are potential targets for CA-based immunotherapy or mRNA vaccine strategies. However, cell-surface proteins are often underrepresented in standard proteomic data sets, due to their poor solubility and lower expression levels compared to intracellular proteins. In this context, we investigated GBM-associated surfaceome by comparison to healthy astrocytes surfaceome to identify new specific targets to GBM. For this purpose, biotinylation of cell surface proteins has been carried out in GBM and healthy astrocytes cell lines. Biotinylated proteins were purified on streptavidin beads and analyzed by shotgun proteomics. After filtering our data with Cell Surface Proteins Atlas (CSPA) and Gene Ontology, 78 overexpressed or exclusive in GBM have been identified. Validation has been performed using Human Protein Atlas. In this context, we identified 21 specific potential targets for GBM including 5 mutated proteins (RELL1, CYBA, EGFR, and MHC I proteins). Taken together, we identified potential targets for immune therapy strategies in GBM.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_04:45:35.277.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Melanie Rose
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	acetylated residue; monohydroxylated residue
Instrument	Q Exactive

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-05 08:55:50	ID requested
⏵ 1	2021-11-03 04:45:35	announced

Dataset with its publication pending

submitter keyword: Glioblastoma, Immunotherapy, Surfaceome, Surface proteins, Mutated proteins

Michel Salzet
contact affiliation	PRISM - Inserm U1192 Universite de Lille France
contact email	michel.salzet@univ-lille.fr
lab head
Melanie Rose
contact affiliation	PRISM Laboratory
contact email	melanie.rose@univ-lille.fr
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD027110
     1. Label: PRIDE project
     2. Name: Surfaceome proteomic of glioblastoma revealed potential targets for immunotherapy