PXD027098-2

PXD027098 is an original dataset announced via ProteomeXchange.

Title	Post-translational responses to elevated c-di-GMP levels in Pseudomonas aeruginosa
Description	To analyze the impact of elevated c-di-GMP concentrations in P. aeruginosa, we expressed pleD* on an inducible vector (pHERD20T) in the PAO1 wild-type strain. PleD is a DGC from Caulobacter cresentusand the pleD* construct variant encodes for a constitutively active enzyme due to four amino acid exchanges (T120N, T214A, P234H, N357Y).We aimed to analyze the cellular consequences of increased c-di-GMP levels in the opportunistic pathogen P. aeruginosa on a global scale. We therefore grew the pleD* harboring PAO1 as well as the empty vector control PAO1 in LB medium, added arabinose (0.2%) to the medium to induce pleD* expression and harvested the cells in exponential growth phase, when the cells exhibited elevated c-di-GMP levels of about two-fold (see manuscript). We are discribing the characteristics of elevated c-di-GMP with a Multi-Omics-Dataset.
HostingRepository	PRIDE
AnnounceDate	2023-11-14
AnnouncementXML	Submission_2023-11-14_08:53:15.908.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julius Ned Witte
SpeciesList	scientific name: Pseudomonas aeruginosa PAO1; NCBI TaxID: 208964;
ModificationList	monohydroxylated residue; deamidated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap Velos

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-05 02:06:55	ID requested
1	2022-07-25 03:06:57	announced
⏵ 2	2023-11-14 08:53:16	announced	2023-11-14: Updated project metadata.

Dataset with its publication pending

submitter keyword: Multi-omics dataset, PleD-overexpression, elevated c-di-GMP levels

Susanne Hauessler
contact affiliation	Institute for Molecular Bacteriology, TWINCORE GmbH, Center of Clinical and Experimental Infection Research, a joint venture of the Hannover Medical School and the Helmholtz Center for Infection Research, Hannover, Germany.
contact email	Susanne.haeussler@helmholtz-hzi.de
lab head
Julius Ned Witte
contact affiliation	TWINCORE GmbH and Proteomics Core Unit MHH
contact email	julius.witte@twincore.de
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/07/PXD027098

PRIDE project URI

• PRIDE
  1. PXD027098
     1. Label: PRIDE project
     2. Name: Post-translational responses to elevated c-di-GMP levels in Pseudomonas aeruginosa