PXD027080-1

PXD027080 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Protein kinase signalling at the Leishmania kinetochore captured by XL-BioID
Description	Elucidating protein kinase driven signaling pathways is an important but challenging problem in cell biology. Phosphoproteomics has been used to identify many phosphorylation sites, however the spatial context of these sites within the cell is mostly unknown making it difficult to reconstruct signalling pathways. To address this problem an in vivo proximity capturing workflow was developed consisting of proximity biotinylation followed by protein cross-linking (XL-BioID). This was applied to protein kinases of the Leishmania kinetochore leading to the discovery of a novel essential kinetochore protein, KKT26. XL-BioID enabled the quantification of proximal phosphosites at the kinetochore through the cell cycle, allowing the phosphorylation state of the kinetochore to be followed during assembly. A specific inhibitor of kinetochore protein kinases KKT10/KKT19 was used to show that XL-BioID provides a spatially focussed view of protein kinase inhibition, identifying 16 inhibitor responsive proximal phosphosites, including 3 on KKT2, demonstrating the potential of this approach for discovery of in vivo kinase signalling pathways.
HostingRepository	MassIVE
AnnounceDate	2021-07-08
AnnouncementXML	Submission_2021-07-08_04:17:41.091.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Adam Dowle
SpeciesList	scientific name: Leishmania mexicana; NCBI TaxID: 5665;
ModificationList	Carbamidomethyl; Oxidation; Phospho; Acetyl; Biotin
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-04 00:31:41	ID requested
⏵ 1	2021-07-08 04:17:41	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: Proximity biotinylation, Proximity labeling, Cross-linking, Kinase, Kinetochore, Leishmania, Phosphorylation, Phosphosite, miniTurboID, BirA, AB1

submitter keyword: Proximity biotinylation, Proximity labeling, Cross-linking, Kinase, Kinetochore, Leishmania, Phosphorylation, Phosphosite, miniTurboID, BirA, AB1

Contact List

Jeremy C. Mottram
contact affiliation	University of York
contact email	jeremy.mottram@york.ac.uk
lab head
Adam Dowle
contact affiliation	University of York
contact email	adam.dowle@york.ac.uk
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087750/