PXD027074 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | FIRST ATTEMPT TO COUPLE PROTEOMIC WITH AhR REPORTER GENE BIOASSAY IN SOIL POLLUTION MONITORING AND ASSESSMENT |
Description | A topsoil sample obtained from a highly industrialized area was tested on CALUX® cell line and exposed cells processed with proteomic and bioinformatics analyses. GC-MS/MS analysis of topsoil extracts was also performed to confirm PCDD, PCDF and dl-PCBs presence. CALUX® and GC-MS/MS data confirmed the presence of PCDD/Fs and dl-PCBs in the topsoil extract. Proteomic analysis of the cells exposed to the soil extracts identified 43 differential proteins. Enrichment analysis highlights biological processes such as cellular response to chemical stimulus, to stress, response to inorganic substance, regulation of translation, regulation of apoptotic process as well as response to organonitrogen compounds in light of particular drugs and compounds, extrapolated by bioinformatics and linked to the identified proteins modifications. These results confirm the complex epidemiological situation occurring among Taranto inhabitants and underline the need to further investigate the presence and sources of inferred chemicals in Taranto soils. The combined analysis reveals a more complex scenarios of chemicals able to affect cellular pathways and leading to toxicities rather than those identified by the bioassay. The approach to couple proteomic to CALUX® is a promising tool and deserves further investigation to be applied in soil pollution monitoring and risk assessment. |
HostingRepository | PRIDE |
AnnounceDate | 2022-02-17 |
AnnouncementXML | Submission_2022-02-17_04:56:20.308.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Enxhi Shaba |
SpeciesList | scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116; |
ModificationList | No PTMs are included in the dataset |
Instrument | autoflex |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-02 08:24:42 | ID requested | |
⏵ 1 | 2022-02-17 04:56:21 | announced | |
Publication List
Landi C, Liberatori G, Cotugno P, Sturba L, Vannuccini ML, Massari F, Miniero DV, Tursi A, Shaba E, Behnisch PA, Carleo A, Di Giuseppe F, Angelucci S, Bini L, Corsi I, Reporter Gene Bioassay in Soil Pollution Monitoring and Assessment. Toxics, 10(1):(2021) [pubmed] |
Keyword List
submitter keyword: DR-CALUX |
Proteomics |
polychlorinated dibenzo-p-dioxins |
polychlorinated dibenzofurans |
dioxin-like polychlorinated biphenyls. |
Contact List
Prof. Luca Bini |
contact affiliation | Functional Proteomics Lab, Life Sciences Department, University of Siena, Italy |
contact email | luca.bini@unisi.it |
lab head | |
Enxhi Shaba |
contact affiliation | University of Siena |
contact email | shaba3@student.unisi.it |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/02/PXD027074 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027074
- Label: PRIDE project
- Name: FIRST ATTEMPT TO COUPLE PROTEOMIC WITH AhR REPORTER GENE BIOASSAY IN SOIL POLLUTION MONITORING AND ASSESSMENT