PXD027073 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | FPB - 2021 - Azri et al - Physiological and proteomic analyses of Tunisian local grapevine cultivar Razegui in response to drought stress. |
Description | Cuttings from Razegui cultivar were cultivated in sand for 2 months according to the method we have described previously (Azri et al. 2020). Then grapevine plantlets were further grown in pots containing sand/peat mixture (2/1, v/v), in a controlled greenhouse (16 h light/8 h dark at PAR 300 μmol m-2 s-1 photoperiod, 24 °C day/20 °C night temperature and 65-75% relative humidity). They were watered daily with tap water and supplemented once a week with 100 mL of Gibeaut’s nutrient solution (Gibeaut et al. 1997). Drought stress was applied according to our previously described procedure (Azri et al. 2020): plants with 10-15 leaves were either irrigated daily with water (control) or non-irrigated at all (water deficit). After 4 time points (day 0, 4, 8 and 16) of water deficit, the 4th fully-developed leaf was harvested and used for physiological analyses. Three independent biological experiments were performed consisting each in 2 treatments (control and water deficit) and 3 plants per time point and treatment. Proteomic analysis was performed on the 5th fully developed leaves at the time point 16 days of drought treatment. Irrigated and non-irrigated leaves were harvested immediately in liquid nitrogen and stored at - 80 °C until analysis. Three biological replicates were carried out for each treatment. |
HostingRepository | PRIDE |
AnnounceDate | 2021-08-06 |
AnnouncementXML | Submission_2021-08-05_21:24:59.429.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | Pascal COSETTE |
SpeciesList | scientific name: Vitis vinifera (Grape); NCBI TaxID: 29760; |
ModificationList | monohydroxylated residue; iodoacetamide derivatized residue |
Instrument | LTQ Orbitrap |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-07-02 08:24:38 | ID requested | |
⏵ 1 | 2021-08-05 21:24:59 | announced | |
Publication List
Dataset with its publication pending |
Keyword List
submitter keyword: Proteome, vitis vinifera, drought stress, 2D electrophoresis |
Contact List
Ahmed Mliki |
contact affiliation | Laboratory of Plant Molecular Physiology, Centre of Biotechnology of Borj Cedria, P.O. Box 901, 2050 Hammam‑Lif, Tunisia. |
contact email | mlikiahm@gmail.com |
lab head | |
Pascal COSETTE |
contact affiliation | UMR6270 CNRS - Université de Rouen, Plateforme Proteomique PISSARO (IRIB) |
contact email | pascal.cosette@univ-rouen.fr |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
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PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD027073
- Label: PRIDE project
- Name: FPB - 2021 - Azri et al - Physiological and proteomic analyses of Tunisian local grapevine cultivar Razegui in response to drought stress.