PXD027060-1

PXD027060 is an original dataset announced via ProteomeXchange.

Dataset Summary

Title	Long-lived mitochondrial cristae proteins in mouse heart and brain
Description	Long-lived proteins (LLPs) have recently emerged as vital components of intracellular structures whose function is coupled to long-term stability. Mitochondria are multifaceted organelles and their function hinges on the efficient proteome renewal and replacement. Here, using metabolic stable isotope labelling of mice combined with mass spectrometry (MS) based proteomic analysis we demonstrate remarkable longevity for a subset of the mitochondrial proteome. We discovered that mitochondrial LLPs (mt-LLPs) can persist for months in tissues harboring long-lived cells, such as brain and heart. Our analysis revealed an enrichment of mt-LLPs within the inner mitochondrial membrane, specifically in the cristae sub-compartment, and demonstrate that the mitochondrial proteome is not turned over in bulk. Pioneering crosslinking experiments revealed that mt-LLPs are spatially restricted and co-preserved within protein OXPHOS complexes with limited subunit exchange throughout their lifetime. This study provides an explanation for the exceptional mitochondrial protein lifetimes and supports the concept that LLPs provide key structural stability to multiple large and dynamic intracellular structures.
HostingRepository	MassIVE
AnnounceDate	2021-07-02
AnnouncementXML	Submission_2021-07-02_07:17:19.811.xml
DigitalObjectIdentifier
ReviewLevel	Non peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Jeffrey N. Savas
SpeciesList	scientific name: Mus musculus; common name: house mouse; NCBI TaxID: 10090;
ModificationList	Carbamidomethyl
Instrument	Orbitrap Fusion

Dataset History

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-02 07:13:30	ID requested
⏵ 1	2021-07-02 07:17:20	announced

Publication List

no publication

no publication

Keyword List

submitter keyword: mitochondria, cristae, long-lived protein, cross-linking,

submitter keyword: mitochondria, cristae, long-lived protein, cross-linking,

Contact List

Jeffrey N. Savas, PhD
contact affiliation	Department of Neurology Northwestern University
contact email	jeffrey.savas@northwestern.edu
lab head
Jeffrey N. Savas
contact affiliation	Northwestern University
contact email	jeffrey.savas@northwestern.edu
dataset submitter

Full Dataset Link List

MassIVE dataset URI
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MassIVE dataset URI

Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://massive.ucsd.edu/MSV000087712/