PXD027049-1

PXD027049 is an original dataset announced via ProteomeXchange.

Title	GM-CSF nitration is a new driver of myeloid suppressor cell activity in tumors
Description	Reactive oxygen species, including RNS, contribute to the control of multiple immune cell functions within the tumor microenvironment (TME). Tumor-infiltrating myeloid cells (TIMs) represent the archetype of tolerogenic cells that actively contribute to dismantle effective immunity against cancer are among the cells most influenced by these molecules. TIMs inhibit T cell functions and promote tumor progression by several mechanisms including the amplification of the oxidative/nitrosative stress within the TME. In tumors, TIM expansion and differentiation is regulated by the granulocyte-macrophage colony-stimulating factor (GM-CSF), which is produced by cancer and immune cells. Nevertheless, the role of GM-CSF in tumors has not yet been fully elucidated. In this study, we show that GM-CSF activity is significantly affected by RNS-triggered post-translational modifications. The nitration of a single tryptophan residue in the sequence of GM-CSF nourishes the expansion of highly immunosuppressive myeloid subsets in tumor-bearing hosts. Importantly, tumors from colorectal cancer patients express higher levels of nitrated tryptophan compared to non-neoplastic tissues. Collectively, our data identify a novel and selective target that can be exploited to remodel the TME and foster protective immunity against cancer.
HostingRepository	PRIDE
AnnounceDate	2021-10-04
AnnouncementXML	Submission_2021-10-04_07:42:56.636.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Giorgio Arrigoni
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090; scientific name: Glycine max; NCBI TaxID: 3847;
ModificationList	nitrated L-tryptophan; monohydroxylated residue; iodoacetamide derivatized residue
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-07-01 23:25:45	ID requested
⏵ 1	2021-10-04 07:42:56	announced

Dataset with its publication pending

submitter keyword: reactive nitrogen species

cytokines

immunosuppression, post-translational modification

TME

Giorgio Arrigoni
contact affiliation	Department of Biomedical Sciences, University of Padova, Italy
contact email	giorgio.arrigoni@unipd.it
lab head
Giorgio Arrigoni
contact affiliation	University of Padova
contact email	giorgio.arrigoni74@gmail.com
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/10/PXD027049

PRIDE project URI

• PRIDE
  1. PXD027049
     1. Label: PRIDE project
     2. Name: GM-CSF nitration is a new driver of myeloid suppressor cell activity in tumors