PXD027026-1

PXD027026 is an original dataset announced via ProteomeXchange.

Title	Two related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through two different acetyltransferase-independent mechanisms
Description	SAGA and ATAC are two related transcriptional coactivator complexes, sharing the same histone acetyltransferase (HAT) subunit. The HAT activities of SAGA and ATAC are required for metazoan development but the precise role of the two complexes in RNA polymerase II transcription in mammals is less understood. To determine whether SAGA and ATAC have redundant or specific functions dependent on their HAT activities, we compared the effects of HAT inactivation in each complex with that of inactivation of either SAGA or ATAC core subunits in mouse embryonic stem cells (ESCs). We show that core subunits of SAGA or ATAC subunits are required for complex assembly, mouse ESC growth and self-renewal. Additionally, ATAC, but not SAGA subunits are required for ESC viability by regulating the transcription of translation-related genes. Surprisingly, depletion of specific or shared HAT module subunits caused a global decrease in histone H3K9 acetylation, but did not result in significant phenotypic or transcriptional defects. Thus, our results indicate that SAGA and ATAC are differentially required for viability and self-renewal of mouse ESCs by regulating transcription through different pathways, in a HAT-independent manner.
HostingRepository	PRIDE
AnnounceDate	2021-08-11
AnnouncementXML	Submission_2021-08-11_05:31:35.322.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Luc Negroni
SpeciesList	scientific name: Mus musculus (Mouse); NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap Elite

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-30 22:39:59	ID requested
⏵ 1	2021-08-11 05:31:35	announced

Dataset with its publication pending

submitter keyword: Mouse embryonic stem cells, coactivator complexes, SAGA, ATAC, Pol II transcription, 4sU labelling, newly synthesized RNA, histone acetyltransferase, HAT-independent function

Didier Devys
contact affiliation	Institut de Génétique et de Biologie Moléculaire et Cellulaire, 67404 Illkirch CEDEX, France
contact email	devys@igbmc.fr
lab head
Luc Negroni
contact affiliation	CNRS
contact email	luc.negroni@igbmc.fr
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/08/PXD027026

PRIDE project URI

• PRIDE
  1. PXD027026
     1. Label: PRIDE project
     2. Name: Two related coactivator complexes SAGA and ATAC control embryonic stem cell self-renewal through two different acetyltransferase-independent mechanisms