PXD026973-1

PXD026973 is an original dataset announced via ProteomeXchange.

Title	Neuronal ribosomes dynamically exchange ribosomal proteins in a context-dependent manner.
Description	Owing to their morphological complexity and dense network connections, neurons modify their proteomes locally, using mRNAs and ribosomes present in the neuropil (tissue enriched for dendrites and axons). Although ribosome biogenesis largely takes place in the nucleus and perinuclear region, neuronal ribosomal protein (RP) mRNAs have been frequently detected remotely, in dendrites and axons. Here, using imaging and ribosome profiling, we directly detected the RP mRNAs and their translation in the neuropil. Combining brief metabolic labeling with mass spectrometry, we found that a group of RPs rapidly associated with translating ribosomes in the cytoplasm and that this incorporation is independent of canonical ribosome biogenesis. Moreover, the incorporation probability of some RPs was regulated by location (neurites vs. cell bodies) and changes in the cellular environment (following oxidative stress). Our results suggest new mechanisms for the local activation, repair and/or specialization of the translational machinery within neuronal processes, potentially allowing neuronal synapses a rapid means to regulate local protein synthesis.
HostingRepository	PRIDE
AnnounceDate	2024-10-07
AnnouncementXML	Submission_2024-10-07_13:40:52.808.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Julian Langer
SpeciesList	scientific name: Rattus norvegicus (Rat); NCBI TaxID: 10116;
ModificationList	6x(13)C; 6x(13)C; iodoacetamide derivatized residue
Instrument	Q Exactive Plus

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-28 07:03:51	ID requested
⏵ 1	2024-10-07 13:40:53	announced

Dataset with its publication pending

submitter keyword: neuronal ribosomes, PRM, rRNA, ribosome assembly, protein complex, stress, H2O2, ribosomal proteins, protein synthesis, translation, local translation, pSILAC, neurons, neuronal processes, SILAC,ribosome, ribosome biogenesis, oxidative stress, hydrogen peroxide

Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@brain.mpg.de
lab head
Julian Langer
contact affiliation	MPIs for Biophysics and Brain Research
contact email	julian.langer@biophys.mpg.de
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026973
     1. Label: PRIDE project
     2. Name: Neuronal ribosomes dynamically exchange ribosomal proteins in a context-dependent manner.