PXD026930-1

PXD026930 is an original dataset announced via ProteomeXchange.

Title	Impact of Alanyl-tRNA Synthetase Editing Deficiency in Yeast
Description	Aminoacyl-tRNA synthetases (aaRSs) are essential enzymes that provide the ribosome with aminoacyl-tRNA substrates for protein synthesis. Mutations in aaRSs lead to various neurological disorders in humans. Many aaRSs utilize editing to prevent error propagation during translation. Editing defects in alanyl-tRNA synthetase (AlaRS) cause neurodegeneration and cardioproteinopathy in mice and is associated with microcephaly in human patients. The cellular impact of AlaRS editing deficiency in eukaryotes remains unclear. Here we use yeast as a model organism to systematically investigate the physiological role of AlaRS editing. Our RNA sequencing and quantitative proteomics analyses reveal that AlaRS editing defects surprisingly activate the general amino acid control pathway and attenuate the heatshock response. We have confirmed these results with reporter and growth assays. In addition, AlaRS editing defects downregulate carbon metabolism and attenuate protein synthesis. Supplying yeast cells with extra carbon source partially rescues the heat sensitivity caused by AlaRS editing deficiency. These findings are in stark contrast with the cellular effects caused by editing deficiency in other aaRSs. Our study therefore highlights the idiosyncratic role of AlaRS editing compared with other aaRSs and provides a model for the physiological impact caused by the lack of AlaRS editing.
HostingRepository	PRIDE
AnnounceDate	2021-08-26
AnnouncementXML	Submission_2021-08-26_05:20:23.035.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Jiqiang Ling
SpeciesList	scientific name: Saccharomyces cerevisiae (Baker's yeast); NCBI TaxID: 4932;
ModificationList	No PTMs are included in the dataset
Instrument	LTQ Orbitrap

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-25 03:54:51	ID requested
⏵ 1	2021-08-26 05:20:23	announced

Dataset with its publication pending

submitter keyword: yeast AlaRS variants, label-free quantitative proteomics

Jiqiang Ling
contact affiliation	Department of Cell Biology and Molecular Genetics University of Maryland
contact email	jling12@umd.edu
lab head
Jiqiang Ling
contact affiliation	University of Maryland
contact email	jling12@umd.edu
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026930
     1. Label: PRIDE project
     2. Name: Impact of Alanyl-tRNA Synthetase Editing Deficiency in Yeast