PXD026918-2

PXD026918 is an original dataset announced via ProteomeXchange.

Title	The therapeutic mechanism of Dengzhanxixin injection on middle cerebral artery occlusion rats based on proteomics
Description	To explore the therapeutic mechanism, we carried out an Dengzhanxixin injection experiment with a focal brain ischemic deficit rat model and explore the target proteins using TMT-based proteomics. Method are shown as follow: 1. Animals and Grouping: The animal experiments were conducted under the direction of NIH Guidelines for the Care and Use of Laboratory Animals (NIH Publications No. 80-23, revised 1996). The procedures were approved by the Animal Care and Use Committees of Beijing Normal University, China. Adult male Sprague–Dawley rats were purchased from Beijing Vital River Laboratory Animal Technology Co., Ltd. The animals were allowed to acclimate for 7 d before the experiments. Rats were randomly divided into a sham-operated (sham) group, model group, Dengzhan-treated (Dengzhanxixin injection) group, and EDV-treated (edaravone, EDV) group. Dengzhan (0.36 mL/100 g) or EDV (0.18 mL/100 g) was intravenously injected into rats, the first drug treatments were performed immediately after the middle cerebral artery occlusion (MCAO) surgery, the subsequent ones were continually performed with the interval of 12 h, i.e. twice a day; and the drug treatments lasted for 7 d. The rats in the sham group and the model group were treated with the same volume of saline in the same way. 2. MCAO Surgery: Before surgery, rats were fasted overnight, with free access to water. Animals were anaesthetized with 3.5% chloral hydrate (350 mg/kg body weight, i.p.) and MCAO was performed. Briefly, the right common carotid artery, external carotid and internal carotid were exposed through a midline cut. A nylon filament with a heated rounded tip was inserted into the external carotid artery, and was pulled to the internal carotid artery to occlude the origin of the middle cerebral artery. Model animals were subject to 1.5 h of MCAO, and then to reperfusion achieved by a gentle withdrawal of the occluding thread. Sham-operated animals were subjected to the same procedure, except for the insertion of the filament.
HostingRepository	iProX
AnnounceDate	2021-06-25
AnnouncementXML	Submission_2023-02-06_19:22:00.533.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Dongfeng Wei
SpeciesList	scientific name: Rattus norvegicus; NCBI TaxID: 10116;
ModificationList	iodoacetamide derivatized residue
Instrument	Orbitrap Fusion Lumos

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-24 19:54:56	ID requested
1	2021-06-24 19:55:23	announced
⏵ 2	2023-02-06 19:22:01	announced	2023-02-07: Update publication information.

An H, Tao W, Liang Y, Li P, Li M, Zhang X, Chen K, Wei D, Xie D, Zhang Z, Dengzhanxixin Injection Ameliorates Cognitive Impairment Through a Neuroprotective Mechanism Based on Mitochondrial Preservation in Patients With Acute Ischemic Stroke. Front Pharmacol, 12():712436(2021) [pubmed]

submitter keyword: MCAO rat, Dengzhanxixin injection, brain ischemic core tissue, TMT proteomics, acute ischemic stroke

Dongfeng Wei
contact affiliation	Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences
contact email	weidongfeng@aliyun.com
lab head
Dongfeng Wei
contact affiliation	Institute of Basic Research in Clinical Medicine, China Academy of Chinese Medical Sciences
contact email	weidongfeng@aliyun.com
dataset submitter

iProX dataset URI