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PXD026880-1

PXD026880 is an original dataset announced via ProteomeXchange.

Dataset Summary
TitleNascent alt-protein chemoproteomics reveals a repressor of ribosome biogenesisNascent alt-protein chemoproteomics reveals a repressor of pre-60S assembly
DescriptionMany unannotated microproteins and alternative proteins (alt-proteins) have recently been found to be co-encoded with canonical proteins, but few of their functions are known. Motivated by the hypothesis that alt-proteins undergoing regulated synthesis could play important cellular roles, we developed a chemoproteomic pipeline to identify nascent alt-proteins in human cells. We identified 22 actively translated alt-proteins or N-terminal extensions, one of which is post-transcriptionally upregulated by DNA damage stress. We further defined cell cycle-regulated MINAS-60 (MIcroprotein that Negatively regulates ASsembly of the pre-60S ribosomal subunit), a nucleolar alt-protein co-encoded with human RBM10. Depletion of MINAS-60 increases the amount of cytoplasmic 60S ribosomal subunit, upregulating global protein synthesis and cell proliferation. Mechanistically, MINAS-60 represses the rate of late-stage pre-60S assembly and export to the cytoplasm. Together, these results implicate MINAS-60 as a repressor of pre-60S maturation, and demonstrate that chemoproteomics can enable functional hypothesis generation for uncharacterized alt-proteins.
HostingRepositoryPRIDE
AnnounceDate2022-09-13
AnnouncementXMLSubmission_2022-09-13_12:53:42.933.xml
DigitalObjectIdentifier
ReviewLevelPeer-reviewed dataset
DatasetOriginOriginal dataset
RepositorySupportUnsupported dataset by repository
PrimarySubmitterxiongwen cao
SpeciesList scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationListNo PTMs are included in the dataset
InstrumentQ Exactive
Dataset History
RevisionDatetimeStatusChangeLog Entry
02021-06-23 08:00:31ID requested
12022-09-13 12:53:44announced
Publication List
Cao X, Khitun A, Harold CM, Bryant CJ, Zheng SJ, Baserga SJ, Slavoff SA, Nascent alt-protein chemoproteomics reveals a pre-60S assembly checkpoint inhibitor. Nat Chem Biol, 18(6):643-651(2022) [pubmed]
Keyword List
submitter keyword: human HEK 293T, LC-MS/MS
Contact List
Sarah A. Slavoff
contact affiliationYale University
contact emailsarah.slavoff@yale.edu
lab head
xiongwen cao
contact affiliationYale University, Chemical Biology Institute
contact emailxiongwen.cao@yale.edu
dataset submitter
Full Dataset Link List
Dataset FTP location
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PRIDE project URI
Repository Record List
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