PXD026880 is an
original dataset announced via ProteomeXchange.
Dataset Summary
Title | Nascent alt-protein chemoproteomics reveals a repressor of ribosome biogenesisNascent alt-protein chemoproteomics reveals a repressor of pre-60S assembly |
Description | Many unannotated microproteins and alternative proteins (alt-proteins) have recently been found to be co-encoded with canonical proteins, but few of their functions are known. Motivated by the hypothesis that alt-proteins undergoing regulated synthesis could play important cellular roles, we developed a chemoproteomic pipeline to identify nascent alt-proteins in human cells. We identified 22 actively translated alt-proteins or N-terminal extensions, one of which is post-transcriptionally upregulated by DNA damage stress. We further defined cell cycle-regulated MINAS-60 (MIcroprotein that Negatively regulates ASsembly of the pre-60S ribosomal subunit), a nucleolar alt-protein co-encoded with human RBM10. Depletion of MINAS-60 increases the amount of cytoplasmic 60S ribosomal subunit, upregulating global protein synthesis and cell proliferation. Mechanistically, MINAS-60 represses the rate of late-stage pre-60S assembly and export to the cytoplasm. Together, these results implicate MINAS-60 as a repressor of pre-60S maturation, and demonstrate that chemoproteomics can enable functional hypothesis generation for uncharacterized alt-proteins. |
HostingRepository | PRIDE |
AnnounceDate | 2022-09-13 |
AnnouncementXML | Submission_2022-09-13_12:53:42.933.xml |
DigitalObjectIdentifier | |
ReviewLevel | Peer-reviewed dataset |
DatasetOrigin | Original dataset |
RepositorySupport | Unsupported dataset by repository |
PrimarySubmitter | xiongwen cao |
SpeciesList | scientific name: Homo sapiens (Human); NCBI TaxID: 9606; |
ModificationList | No PTMs are included in the dataset |
Instrument | Q Exactive |
Dataset History
Revision | Datetime | Status | ChangeLog Entry |
0 | 2021-06-23 08:00:31 | ID requested | |
⏵ 1 | 2022-09-13 12:53:44 | announced | |
Publication List
Cao X, Khitun A, Harold CM, Bryant CJ, Zheng SJ, Baserga SJ, Slavoff SA, Nascent alt-protein chemoproteomics reveals a pre-60S assembly checkpoint inhibitor. Nat Chem Biol, 18(6):643-651(2022) [pubmed] |
Keyword List
submitter keyword: human HEK 293T, LC-MS/MS |
Contact List
Sarah A. Slavoff |
contact affiliation | Yale University |
contact email | sarah.slavoff@yale.edu |
lab head | |
xiongwen cao |
contact affiliation | Yale University, Chemical Biology Institute |
contact email | xiongwen.cao@yale.edu |
dataset submitter | |
Full Dataset Link List
Dataset FTP location
NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/09/PXD026880 |
PRIDE project URI |
Repository Record List
[ + ]
[ - ]
- PRIDE
- PXD026880
- Label: PRIDE project
- Name: Nascent alt-protein chemoproteomics reveals a repressor of ribosome biogenesisNascent alt-protein chemoproteomics reveals a repressor of pre-60S assembly