PXD026859-1

PXD026859 is an original dataset announced via ProteomeXchange.

Title	Proteomic analysis of filaggrin insufficient keratinocytes and secreted exosomes.
Description	Filaggrin (FLG) is a key structural protein expressed in epidermal keratinocytes. Mutations in the filaggrin gene (FLG) are strongly linked to atopic dermatitis (AD) and allergic inflammation occurring later in life at distant body locations in AD patients. Keratinocytes secrete exosomes, small, lipid-rich membrane vesicles to communicate with distant cells, including within the immune system. The FLG gene was silenced in the HaCaT keratinocyte cell line by shRNA delivered using a lentiviral vector. HaCaT cells transduced with scrambled shRNA were used as a control.
HostingRepository	PRIDE
AnnounceDate	2022-08-12
AnnouncementXML	Submission_2022-08-12_06:44:56.388.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	Aleksandra Lewandowska
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	No PTMs are included in the dataset
Instrument	TripleTOF 5600

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-22 02:53:50	ID requested
⏵ 1	2022-08-12 06:44:56	announced

Dataset with its publication pending

submitter keyword: keratinocyte, filaggrin, atopic dermatitis, ichthyosis vulgaris, exosomes, CD1a, LC-MS/MS

Paulina Czaplewska
contact affiliation	Laboratory of Mass Spectrometry, Core Facility Laboratories, Intercollegiate Faculty of Biotechnology UG-MUG, University of Gdansk, Gdansk, Poland
contact email	paulina.czaplewska@ug.edu.pl
lab head
Aleksandra Lewandowska
contact affiliation	University of Gdansk
contact email	aleksandra.lewandowska@biotech.ug.edu.pl
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2022/08/PXD026859

PRIDE project URI

• PRIDE
  1. PXD026859
     1. Label: PRIDE project
     2. Name: Proteomic analysis of filaggrin insufficient keratinocytes and secreted exosomes.