PXD026821-1

PXD026821 is an original dataset announced via ProteomeXchange.

Title	Integrated liver and plasma proteomics in obese mice reveals complex metabolic and immune regulation
Description	Obesity leads to the development of non-alcoholic fatty liver disease (NAFLD) and associated alterations to the plasma proteome. To elucidate the underlying changes associated with obesity, we performed liquid chromatography-tandem mass spectrometry in the liver and plasma of obese leptin-deficient ob/ob mice. We quantified 7173 and 555 proteins in the liver and plasma, respectively. The abundance of proteins related to fatty acid metabolism were increased, alongside peroxisomal proliferation in ob/ob liver. Putatively secreted proteins and the secretory machinery were also dysregulated in the liver, which was mirrored by a substantial alteration of the plasma proteome. Greater than 50% of the plasma proteins were differentially regulated, including NAFLD biomarkers, lipoproteins, the 20S proteasome, and the complement and coagulation cascades of the immune system. Integration of the proteomes identified proteins that were concomitantly regulated in the liver and plasma in obesity, suggesting the systemic abundance of these plasma proteins are regulated by secretion from the liver. These analyses yield a comprehensive insight into obesity and provide an extensive resource for obesity research in a prevailing model organism.
HostingRepository	PRIDE
AnnounceDate	2022-03-01
AnnouncementXML	Submission_2022-03-01_00:11:54.350.xml
DigitalObjectIdentifier
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Unsupported dataset by repository
PrimarySubmitter	atul shahaji deshmukh
SpeciesList	scientific name: Mus musculus; NCBI TaxID: 10090;
ModificationList	No PTMs are included in the dataset
Instrument	Q Exactive HF

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-20 22:54:36	ID requested
⏵ 1	2022-03-01 00:11:54	announced
2	2023-11-14 08:53:08	announced	2023-11-14: Updated project metadata.

10.1016/J.MCPRO.2022.100207;

submitter keyword: LCMS, Proteome, NAFLD, Obesity, ob/ob

Atul S Deshmukh
contact affiliation	Novo Nordisk Center For Basic Metabolic Research, University of Copenhagen.
contact email	atul.deshmukh@sund.ku.dk
lab head
atul shahaji deshmukh
contact affiliation	Copenhagen University
contact email	atul.deshmukh@cpr.ku.dk
dataset submitter

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PRIDE project URI

• PRIDE
  1. PXD026821
     1. Label: PRIDE project
     2. Name: Integrated liver and plasma proteomics in obese mice reveals complex metabolic and immune regulation