PXD026815-1

PXD026815 is an original dataset announced via ProteomeXchange.

Title	SARS_CoV-2 substrate profiling in interferon-treated BEAS-2B cell proteomes
Description	To profile the human substrate degradome of the SARS-CoV-2 main viral protease and to investigate whether 3CLpro cleavage of cellular substrates dampens host antiviral responses induced by type I interferons, we treated BEAS-2B cells with interferon-alpha (N = 3), interferon-beta (N = 3), or vehicle (N = 3), retrieve and exposed native proteome extracts from human embryonic kidney to 3CLpro cleavage. Then, N Terminal Amine Labeling of Substates (TAILS) was used to identify the substrates. Here, heavy [+34] isotope dimethyl labeling is performed at the whole protein level in the 3CLpro-exposed samples, and the P1' side of substrates substrates was revealed by comparing to the samples exposed to inactive mutant labeled with ligh [+28 Da] dimethyl.
HostingRepository	PRIDE
AnnounceDate	2021-11-03
AnnouncementXML	Submission_2021-11-03_05:54:57.579.xml
DigitalObjectIdentifier	https://dx.doi.org/10.6019/PXD026815
ReviewLevel	Peer-reviewed dataset
DatasetOrigin	Original dataset
RepositorySupport	Supported dataset by repository
PrimarySubmitter	Yoan Machado
SpeciesList	scientific name: Homo sapiens (Human); NCBI TaxID: 9606;
ModificationList	N-ethylmaleimide derivatized cysteine; 2-pyrrolidone-5-carboxylic acid (Gln); acetylated residue; monohydroxylated residue
Instrument	maXis

Revision	Datetime	Status	ChangeLog Entry
0	2021-06-20 22:39:43	ID requested
⏵ 1	2021-11-03 05:54:57	announced

Dataset with its publication pending

ProteomeXchange project tag: Sars-cov-2, Covid-19

submitter keyword: SARS-CoV-2, TAILS, 3CLpro, Interferon, lung, Bruker Q-TOF, Byonic

Christopher M. Overall
contact affiliation	Center for Blood Research, Life Sciences Centre, University of British Columbia; Vancouver, British Columbia, Canada, V6T 1Z3.
contact email	chris.overall@ubc.ca
lab head
Yoan Machado
contact affiliation	University of British Columbia
contact email	yoan.machado@ubc.ca
dataset submitter

Dataset FTP location NOTE: Most web browsers have now discontinued native support for FTP access within the browser window. But you can usually install another FTP app (we recommend FileZilla) and configure your browser to launch the external application when you click on this FTP link. Or otherwise, launch an app that supports FTP (like FileZilla) and use this address: ftp://ftp.pride.ebi.ac.uk/pride/data/archive/2021/11/PXD026815

PRIDE project URI

• PRIDE
  1. PXD026815
     1. Label: PRIDE project
     2. Name: SARS_CoV-2 substrate profiling in interferon-treated BEAS-2B cell proteomes